Expression analysis of MIF4GD in the rat testis

Kenzo Okada, Masanori Kimura, Yusuke Moriyama, Michiko Nakai, Kazuhiro Kikuchi, Hiroyuki Kaneko, Tetsuo Kunieda, Tadashi Baba, Junko Noguchi

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Abstract

The difference between transcriptional and translational activities of genes is one of the remarkable features in spermatogenesis. In this study, we characterized MIF4GD (MIF4G domain containing), a possible translational regulator, to uncover the function during rat spermatogenesis. Western blot analysis revealed the presence of two forms of MIF4GD with molecular sizes of 25 and 16 kDa in various tissues including the testis. An additional 20-kDa form of MIF4GD was also found exclusively in the testis. These three proteins were abundantly present in pachytene spermatocytes and haploid spermatids. Importantly, the 16-kDa MIF4GD was distinguished from the 25- and 20-kDa proteins by presence in fractions of 40S subunits and ribosomes of spermatogenic cells according to analysis of subcellular localizations. These results suggest that the 16-kDa MIF4GD may function as a translational regulator in spermiogenesis.

Original languageEnglish
Pages (from-to)256-261
Number of pages6
JournalJournal of Reproduction and Development
Volume57
Issue number2
DOIs
Publication statusPublished - Apr 1 2011

Keywords

  • Rat
  • Spermatogenesis
  • Translation regulation

ASJC Scopus subject areas

  • Animal Science and Zoology

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  • Cite this

    Okada, K., Kimura, M., Moriyama, Y., Nakai, M., Kikuchi, K., Kaneko, H., Kunieda, T., Baba, T., & Noguchi, J. (2011). Expression analysis of MIF4GD in the rat testis. Journal of Reproduction and Development, 57(2), 256-261. https://doi.org/10.1262/jrd.10-138H