Expression analysis of microRNAs in murine cochlear explants

Misato Hirai; Yukihide Maeda; Kunihiro Fukushima; Akiko Sugaya; Yuko Kataoka; Kazunori Nishizaki

doi:10.1097/WNR.0b013e32834a0273

Expression analysis of microRNAs in murine cochlear explants

Misato Hirai, Yukihide Maeda, Kunihiro Fukushima, Akiko Sugaya, Yuko Kataoka, Kazunori Nishizaki

University Hospital

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) play functional roles in sound transduction in cochlea. This study focuses on the validity of cochlear culture as an in vitro experimental tool, in view of miRNA expression. E15 cochleae were dissected and maintained in vitro for 48 h before extraction of miRNAs. MiRNA expression was comprehensively screened in explanted cochleae using a miRNA array that covers 380 miRNAs. A strong correlation was observed between expression levels of miRNAs in in vitro and in in vivo cochleae. Levels of 43 miRNAs were altered in vitro and these changes were reproducible over three trials. These findings indicate that in vitro miRNA profiling is a viable method for analysis of gene expression and action of chemical compounds on cochleae.

Original language	English
Pages (from-to)	652-654
Number of pages	3
Journal	NeuroReport
Volume	22
Issue number	13
DOIs	https://doi.org/10.1097/WNR.0b013e32834a0273
Publication status	Published - Sept 14 2011

Keywords

embryonic cochlea
microRNA array
organ culture of cochlea

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/WNR.0b013e32834a0273

Cite this

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abstract = "MicroRNAs (miRNAs) play functional roles in sound transduction in cochlea. This study focuses on the validity of cochlear culture as an in vitro experimental tool, in view of miRNA expression. E15 cochleae were dissected and maintained in vitro for 48 h before extraction of miRNAs. MiRNA expression was comprehensively screened in explanted cochleae using a miRNA array that covers 380 miRNAs. A strong correlation was observed between expression levels of miRNAs in in vitro and in in vivo cochleae. Levels of 43 miRNAs were altered in vitro and these changes were reproducible over three trials. These findings indicate that in vitro miRNA profiling is a viable method for analysis of gene expression and action of chemical compounds on cochleae.",

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AU - Hirai, Misato

AU - Maeda, Yukihide

AU - Fukushima, Kunihiro

AU - Sugaya, Akiko

AU - Kataoka, Yuko

AU - Nishizaki, Kazunori

PY - 2011/9/14

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AB - MicroRNAs (miRNAs) play functional roles in sound transduction in cochlea. This study focuses on the validity of cochlear culture as an in vitro experimental tool, in view of miRNA expression. E15 cochleae were dissected and maintained in vitro for 48 h before extraction of miRNAs. MiRNA expression was comprehensively screened in explanted cochleae using a miRNA array that covers 380 miRNAs. A strong correlation was observed between expression levels of miRNAs in in vitro and in in vivo cochleae. Levels of 43 miRNAs were altered in vitro and these changes were reproducible over three trials. These findings indicate that in vitro miRNA profiling is a viable method for analysis of gene expression and action of chemical compounds on cochleae.

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KW - microRNA array

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Expression analysis of microRNAs in murine cochlear explants

Abstract

Keywords

ASJC Scopus subject areas

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