Expression analysis of microRNAs in murine cochlear explants

Misato Hirai, Yukihide Maeda, Kunihiro Fukushima, Akiko Sugaya, Yuko Kataoka, Kazunori Nishizaki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) play functional roles in sound transduction in cochlea. This study focuses on the validity of cochlear culture as an in vitro experimental tool, in view of miRNA expression. E15 cochleae were dissected and maintained in vitro for 48 h before extraction of miRNAs. MiRNA expression was comprehensively screened in explanted cochleae using a miRNA array that covers 380 miRNAs. A strong correlation was observed between expression levels of miRNAs in in vitro and in in vivo cochleae. Levels of 43 miRNAs were altered in vitro and these changes were reproducible over three trials. These findings indicate that in vitro miRNA profiling is a viable method for analysis of gene expression and action of chemical compounds on cochleae.

Original languageEnglish
Pages (from-to)652-654
Number of pages3
JournalNeuroReport
Volume22
Issue number13
DOIs
Publication statusPublished - Sep 14 2011

Keywords

  • embryonic cochlea
  • microRNA array
  • organ culture of cochlea

ASJC Scopus subject areas

  • Neuroscience(all)

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