Exposure to negatively charged-particle dominant air-conditions on human lymphocytes in vitro activates immunological responses

Yasumitsu Nishimura, Kazuaki Takahashi, Akinori Mase, Muneo Kotani, Kazuhisa Ami, Megumi Maeda, Takashi Shirahama, Suni Lee, Hidenori Matsuzaki, Naoko Kumagai-Takei, Kei Yoshitome, Takemi Otsuki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Indoor air-conditions may play an important role in human health. Investigation of house conditions that promote health revealed that negatively charged-particle dominant indoor air-conditions (NAC) induced immune stimulation. NAC was established using fine charcoal powder on walls and ceilings and utilizing forced negatively charged particles (approximate diameter: 20. nm) dominant in indoor air-conditions created by applying an electric voltage (72. V) between the backside of the walls and the ground. We reported previously that these conditions induced a slight and significant increase of interleukin-2 during 2.5. h stay, and an increase of natural killer (NK) cell cytotoxicity, when examining human subjects after a two-week night stay under these conditions. In the present study, we investigated whether exposure to NAC in vitro affects immune conditions. Although the concentrations of particles were different, an incubator for cell culture with NAC was set and cellular compositions and functions of various freshly isolated human lymphocytes derived from healthy donors were assayed in the NAC incubator and compared with those of cultures in a standard (STD) incubator. Results showed that NAC cultivation caused an increase of CD25 and PD-1 expressing cells in the CD4 positive fraction, enhancement of NK cell cytotoxicity, production of interferon-y (IFNγ), and slight enhancement of regulatory T cell function. In addition, the formula designated as the "immune-index" clearly differed between STD and NAC culture conditions. Thus, NAC conditions may promote human health through slight activation of the immune system against cancer cells and virus infection as shown by this in vitro study and our previously reported human studies.

Original languageEnglish
Pages (from-to)1359-1368
Number of pages10
JournalImmunobiology
Volume220
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

Incubators
Air
Lymphocytes
Natural Killer Cells
Health
Charcoal
Virus Diseases
Regulatory T-Lymphocytes
Powders
Interferons
Interleukin-2
Immune System
Cell Culture Techniques
In Vitro Techniques
Neoplasms

Keywords

  • Immune Stimulation
  • Indoor air
  • Negatively-charged particle
  • NK cell
  • Regulatory T cell
  • T cell

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology

Cite this

Exposure to negatively charged-particle dominant air-conditions on human lymphocytes in vitro activates immunological responses. / Nishimura, Yasumitsu; Takahashi, Kazuaki; Mase, Akinori; Kotani, Muneo; Ami, Kazuhisa; Maeda, Megumi; Shirahama, Takashi; Lee, Suni; Matsuzaki, Hidenori; Kumagai-Takei, Naoko; Yoshitome, Kei; Otsuki, Takemi.

In: Immunobiology, Vol. 220, No. 12, 01.12.2015, p. 1359-1368.

Research output: Contribution to journalArticle

Nishimura, Y, Takahashi, K, Mase, A, Kotani, M, Ami, K, Maeda, M, Shirahama, T, Lee, S, Matsuzaki, H, Kumagai-Takei, N, Yoshitome, K & Otsuki, T 2015, 'Exposure to negatively charged-particle dominant air-conditions on human lymphocytes in vitro activates immunological responses', Immunobiology, vol. 220, no. 12, pp. 1359-1368. https://doi.org/10.1016/j.imbio.2015.07.006
Nishimura, Yasumitsu ; Takahashi, Kazuaki ; Mase, Akinori ; Kotani, Muneo ; Ami, Kazuhisa ; Maeda, Megumi ; Shirahama, Takashi ; Lee, Suni ; Matsuzaki, Hidenori ; Kumagai-Takei, Naoko ; Yoshitome, Kei ; Otsuki, Takemi. / Exposure to negatively charged-particle dominant air-conditions on human lymphocytes in vitro activates immunological responses. In: Immunobiology. 2015 ; Vol. 220, No. 12. pp. 1359-1368.
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