Export of a Vibrio parahaemolyticus toxin by the Sec and type III secretion machineries in tandem

Shigeaki Matsuda, Ryu Okada, Sarunporn Tandhavanant, Hirotaka Hiyoshi, Kazuyoshi Goto, Tetsuya Iida, Toshio Kodama

Research output: Contribution to journalLetter

1 Citation (Scopus)

Abstract

Many Gram-negative pathogens utilize dedicated secretion systems to export virulence factors such as exotoxins and effectors 1–4 . Several exotoxins are synthesized as precursors containing amino-terminal Sec signal peptides and are exported through the inner-membrane-bound Sec machinery to the periplasm, followed by secretion across the outer membrane to the exterior using a type II secretion system (T2SS) 3,5 . Here, we report that thermostable direct haemolysin (TDH), an exotoxin of the food-borne pathogen Vibrio parahaemolyticus, can be exported through the type III secretion system (T3SS), which engages in one-step secretion of effectors 4 , despite possessing a Sec signal peptide and being mainly secreted via the T2SS. Although the precursor of TDH is targeted to the Sec pathway, a fraction of mature TDH was observed to re-enter the bacterial cytoplasm. The N terminus of mature TDH comprises a T3SS signal sequence, allowing it to be loaded into the T3SS. We also show that T3SS-delivered TDH as an effector contributes to intestinal fluid accumulation in a rabbit diarrhoeal model of V. parahaemolyticus infection. Thus, our results show that an unconventional export mechanism for a bacterial toxin via the T3SS in tandem with the Sec machinery facilitates the virulence trait of V. parahaemolyticus.

Original languageEnglish
JournalNature Microbiology
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Vibrio parahaemolyticus
Exotoxins
Protein Sorting Signals
Vibrio Infections
Bacterial Toxins
Periplasm
Membranes
Virulence Factors
Virulence
Cytoplasm
thermostable direct hemolysin
Rabbits
Food

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Microbiology (medical)
  • Cell Biology

Cite this

Export of a Vibrio parahaemolyticus toxin by the Sec and type III secretion machineries in tandem. / Matsuda, Shigeaki; Okada, Ryu; Tandhavanant, Sarunporn; Hiyoshi, Hirotaka; Goto, Kazuyoshi; Iida, Tetsuya; Kodama, Toshio.

In: Nature Microbiology, 01.01.2019.

Research output: Contribution to journalLetter

Matsuda, Shigeaki ; Okada, Ryu ; Tandhavanant, Sarunporn ; Hiyoshi, Hirotaka ; Goto, Kazuyoshi ; Iida, Tetsuya ; Kodama, Toshio. / Export of a Vibrio parahaemolyticus toxin by the Sec and type III secretion machineries in tandem. In: Nature Microbiology. 2019.
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