Experimentally induced acute hyperinsulinemia stimulates endogenous nitric oxide production in humans: Detection using urinary NO2-/NO3- excretion

Hirokazu Tsukahara, Kiyoshi Kikuchi, Kumi Tsumura, Kouki Kimura, Ikue Hata, Masahiro Hiraoka, Masakatsu Sudo

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Insulin-mediated glucose metabolism in skeletal muscle is associated with a proportional increase in muscle perfusion. The vasodilatory effect of insulin is thought to be mediated in part by endothelium-derived nitric oxide (NO). The present study was performed to determine whether acute hyperinsulinemia has any stimulatory effect on endogenous NO production in humans. Bolus intravenous injection of insulin (0.1 IU/kg body weight) caused a significant increase in urinary excretion of NO2-/NO3-together with s significant decrease in blood pressure, whereas saline infusion alone had no effect on these parameters. The increased NO response to insulin was almost comparable to that obtained with infusion of 30 g L-arginine. The acute effect of hyperinsulinemia on endogenous NO formation supports the concept that NO may mediate the vasodilatory action of insulin in humans.

Original languageEnglish
Pages (from-to)406-409
Number of pages4
JournalMetabolism: Clinical and Experimental
Issue number4
Publication statusPublished - Jan 1 1997
Externally publishedYes


ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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