Experimental pulmonary granuloma mimicking sarcoidosis induced by propionibacterium acnes in mice

Kouji Iio, Tomoe Iio, Yuhei Okui, Hirohisa Ichikawa, Yasushi Tanimoto, Nobuaki Miyahara, Arihiko Kanehiro, Mitsune Tanimoto, Yasunari Nakata, Mikio Kataoka

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Propionibacterium acnes has been implicated as an etiologic agent of sarcoidosis since the isolation of this bacterium from sarcoid lesions. We experimentally produced a murine pulmonary granuloma model using P. acnes with several features that simulate sarcoidosis. Mice were sensitized with heat-killed P. acnes and complete Freund's adjuvant and were subsequently challenged with heat-killed P. acnes at 2-week intervals. P. acnes-challenged mice developed epitheloid cell granulomas in the lungs. These mice showed a pulmonary immune response characterized by an increased number of T-lymphocytes, especially CD4+ cells, and the ratio of CD4+/CD8+ in bronchoalveolar lavage (BAL) fluid also increased. Furthermore, significant elevations in both angiotensin-converting enzyme (ACE) serum levels and antibody titers against P. acnes were observed. Mice sensitized with P. acnes without complete Freund's adjuvant were capable of forming pulmonary granulomas, which appeared to be caused by indigenous P. acnes. The genome of P. acnes was found in the lungs, BAL cells, hilar lymph nodes, liver, and spleen in non-sensitized mice, which were thought to be germ-free. These results suggest that the immune response against indigenous P. acnes may play an important role in the pathogenesis of granuloma formation in a murine model.

Original languageEnglish
Pages (from-to)75-83
Number of pages9
JournalActa Medica Okayama
Volume64
Issue number2
Publication statusPublished - Apr 2010

Fingerprint

Propionibacterium acnes
Freund's Adjuvant
Sarcoidosis
Granuloma
Lung
T-cells
Peptidyl-Dipeptidase A
Liver
Bacteria
Genes
Fluids
Antibodies
Bronchoalveolar Lavage
Hot Temperature
CD4-CD8 Ratio
Bronchoalveolar Lavage Fluid
Spleen
Lymph Nodes
Genome
T-Lymphocytes

Keywords

  • Experimental granuloma
  • Propionibacterium acnes
  • Sarcoidosis

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Experimental pulmonary granuloma mimicking sarcoidosis induced by propionibacterium acnes in mice. / Iio, Kouji; Iio, Tomoe; Okui, Yuhei; Ichikawa, Hirohisa; Tanimoto, Yasushi; Miyahara, Nobuaki; Kanehiro, Arihiko; Tanimoto, Mitsune; Nakata, Yasunari; Kataoka, Mikio.

In: Acta Medica Okayama, Vol. 64, No. 2, 04.2010, p. 75-83.

Research output: Contribution to journalArticle

Iio, K, Iio, T, Okui, Y, Ichikawa, H, Tanimoto, Y, Miyahara, N, Kanehiro, A, Tanimoto, M, Nakata, Y & Kataoka, M 2010, 'Experimental pulmonary granuloma mimicking sarcoidosis induced by propionibacterium acnes in mice', Acta Medica Okayama, vol. 64, no. 2, pp. 75-83.
Iio, Kouji ; Iio, Tomoe ; Okui, Yuhei ; Ichikawa, Hirohisa ; Tanimoto, Yasushi ; Miyahara, Nobuaki ; Kanehiro, Arihiko ; Tanimoto, Mitsune ; Nakata, Yasunari ; Kataoka, Mikio. / Experimental pulmonary granuloma mimicking sarcoidosis induced by propionibacterium acnes in mice. In: Acta Medica Okayama. 2010 ; Vol. 64, No. 2. pp. 75-83.
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