Experimental phage therapy against lethal lung-derived septicemia caused by Staphylococcus aureus in mice

Iyo Takemura-Uchiyama; Jumpei Uchiyama; Makoto Osanai; Norihito Morimoto; Tadashi Asagiri; Takako Ujihara; Masanori Daibata; Tetsuro Sugiura; Shigenobu Matsuzaki

doi:10.1016/j.micinf.2014.02.011

Experimental phage therapy against lethal lung-derived septicemia caused by Staphylococcus aureus in mice

Iyo Takemura-Uchiyama, Jumpei Uchiyama, Makoto Osanai, Norihito Morimoto, Tadashi Asagiri, Takako Ujihara, Masanori Daibata, Tetsuro Sugiura, Shigenobu Matsuzaki

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

Abstract

Nosocomial respiratory infections caused by methicillin-resistant Staphylococcus aureus (MRSA) can progress to lethal systemic infections. Bacteriophage (phage) therapy is expected to be effective against these critical infections. Previously, phage S13' was proposed as a potential therapeutic phage. We here examined phage treatment in a mouse model of lung-derived septicemia using phage S13'. Intraperitoneal phage administration at 6h postinfection reduced the severity of infection and rescued the infected mice. Phage S13' can efficiently lyse hospital-acquired MRSA strains causing pneumonia-associated bacteremia invitro. Thus, phage therapy may be a possible therapeutic intervention in staphylococcal lung-derived septicemia.

Original language	English
Pages (from-to)	512-517
Number of pages	6
Journal	Microbes and Infection
Volume	16
Issue number	6
DOIs	https://doi.org/10.1016/j.micinf.2014.02.011
Publication status	Published - Jun 2014
Externally published	Yes

Keywords

Bacteriophage
Mouse
Podoviridae
Therapeutic
Therapy

ASJC Scopus subject areas

Microbiology
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.micinf.2014.02.011

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title = "Experimental phage therapy against lethal lung-derived septicemia caused by Staphylococcus aureus in mice",

abstract = "Nosocomial respiratory infections caused by methicillin-resistant Staphylococcus aureus (MRSA) can progress to lethal systemic infections. Bacteriophage (phage) therapy is expected to be effective against these critical infections. Previously, phage S13' was proposed as a potential therapeutic phage. We here examined phage treatment in a mouse model of lung-derived septicemia using phage S13'. Intraperitoneal phage administration at 6h postinfection reduced the severity of infection and rescued the infected mice. Phage S13' can efficiently lyse hospital-acquired MRSA strains causing pneumonia-associated bacteremia invitro. Thus, phage therapy may be a possible therapeutic intervention in staphylococcal lung-derived septicemia.",

keywords = "Bacteriophage, Mouse, Podoviridae, Therapeutic, Therapy",

author = "Iyo Takemura-Uchiyama and Jumpei Uchiyama and Makoto Osanai and Norihito Morimoto and Tadashi Asagiri and Takako Ujihara and Masanori Daibata and Tetsuro Sugiura and Shigenobu Matsuzaki",

note = "Funding Information: This study was funded by a Grant-in-Aid for Young Scientists (B) ( 24791025 ), the Center for Innovative and Translational Medicine , Kochi System Glycobiology Center , and the Center of Biomembrane Functions Controlling Biological Systems, Kochi University, Kochi, Japan . ",

year = "2014",

month = jun,

doi = "10.1016/j.micinf.2014.02.011",

language = "English",

volume = "16",

pages = "512--517",

journal = "Microbes and Infection",

issn = "1286-4579",

publisher = "Elsevier Masson SAS",

number = "6",

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TY - JOUR

T1 - Experimental phage therapy against lethal lung-derived septicemia caused by Staphylococcus aureus in mice

AU - Takemura-Uchiyama, Iyo

AU - Uchiyama, Jumpei

AU - Osanai, Makoto

AU - Morimoto, Norihito

AU - Asagiri, Tadashi

AU - Ujihara, Takako

AU - Daibata, Masanori

AU - Sugiura, Tetsuro

AU - Matsuzaki, Shigenobu

N1 - Funding Information: This study was funded by a Grant-in-Aid for Young Scientists (B) ( 24791025 ), the Center for Innovative and Translational Medicine , Kochi System Glycobiology Center , and the Center of Biomembrane Functions Controlling Biological Systems, Kochi University, Kochi, Japan .

PY - 2014/6

Y1 - 2014/6

N2 - Nosocomial respiratory infections caused by methicillin-resistant Staphylococcus aureus (MRSA) can progress to lethal systemic infections. Bacteriophage (phage) therapy is expected to be effective against these critical infections. Previously, phage S13' was proposed as a potential therapeutic phage. We here examined phage treatment in a mouse model of lung-derived septicemia using phage S13'. Intraperitoneal phage administration at 6h postinfection reduced the severity of infection and rescued the infected mice. Phage S13' can efficiently lyse hospital-acquired MRSA strains causing pneumonia-associated bacteremia invitro. Thus, phage therapy may be a possible therapeutic intervention in staphylococcal lung-derived septicemia.

AB - Nosocomial respiratory infections caused by methicillin-resistant Staphylococcus aureus (MRSA) can progress to lethal systemic infections. Bacteriophage (phage) therapy is expected to be effective against these critical infections. Previously, phage S13' was proposed as a potential therapeutic phage. We here examined phage treatment in a mouse model of lung-derived septicemia using phage S13'. Intraperitoneal phage administration at 6h postinfection reduced the severity of infection and rescued the infected mice. Phage S13' can efficiently lyse hospital-acquired MRSA strains causing pneumonia-associated bacteremia invitro. Thus, phage therapy may be a possible therapeutic intervention in staphylococcal lung-derived septicemia.

KW - Bacteriophage

KW - Mouse

KW - Podoviridae

KW - Therapeutic

KW - Therapy

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U2 - 10.1016/j.micinf.2014.02.011

DO - 10.1016/j.micinf.2014.02.011

M3 - Article

C2 - 24631574

AN - SCOPUS:84902772230

SN - 1286-4579

VL - 16

SP - 512

EP - 517

JO - Microbes and Infection

JF - Microbes and Infection

IS - 6

ER -

Experimental phage therapy against lethal lung-derived septicemia caused by Staphylococcus aureus in mice

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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