Abstract
This report describes a new experimental procedure, a rat unilateral, orthotopic lung transplantation with cold storage, and evaluates its relevancy and reliability to study the early events during cold ischemia/reperfusion (I/R) injury. This model, using the cuff technique, does not require extensive training and is relatively easy to be established. The model can induce reproducible degrees of pulmonary graft injury including impaired gas exchange, proinflammatory cytokine upregulation, or inflammatory infiltrates, depending on the preservation time. The results are consistent with the previous clinical evidence, thus suggesting that this model is a valid and reliable animal model of cold I/R injury.
| Original language | English |
|---|---|
| Pages (from-to) | 641-645 |
| Number of pages | 5 |
| Journal | Surgery Today |
| Volume | 39 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 2009 |
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Keywords
- Cold preservation
- Experimental animal model
- Ischemia/reperfusion
- Lung transplantation
ASJC Scopus subject areas
- Surgery
Cite this
Experimental orthotopic lung transplantation model in rats with cold storage. / Sugimoto, Ryujiro; Nakao, Atsunori; Nagahiro, Itaru; Kohmoto, Junichi; Sugimoto, Seiichiro; Okazaki, Mikio; Yamane, Masaomi; Inokawa, Hidetoshi; Oto, Takahiro; Tahara, Kazunori; Zhan, Jianghua; Sano, Yoshifumi; McCurry, Kenneth R.
In: Surgery Today, Vol. 39, No. 7, 07.2009, p. 641-645.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Experimental orthotopic lung transplantation model in rats with cold storage
AU - Sugimoto, Ryujiro
AU - Nakao, Atsunori
AU - Nagahiro, Itaru
AU - Kohmoto, Junichi
AU - Sugimoto, Seiichiro
AU - Okazaki, Mikio
AU - Yamane, Masaomi
AU - Inokawa, Hidetoshi
AU - Oto, Takahiro
AU - Tahara, Kazunori
AU - Zhan, Jianghua
AU - Sano, Yoshifumi
AU - McCurry, Kenneth R.
PY - 2009/7
Y1 - 2009/7
N2 - This report describes a new experimental procedure, a rat unilateral, orthotopic lung transplantation with cold storage, and evaluates its relevancy and reliability to study the early events during cold ischemia/reperfusion (I/R) injury. This model, using the cuff technique, does not require extensive training and is relatively easy to be established. The model can induce reproducible degrees of pulmonary graft injury including impaired gas exchange, proinflammatory cytokine upregulation, or inflammatory infiltrates, depending on the preservation time. The results are consistent with the previous clinical evidence, thus suggesting that this model is a valid and reliable animal model of cold I/R injury.
AB - This report describes a new experimental procedure, a rat unilateral, orthotopic lung transplantation with cold storage, and evaluates its relevancy and reliability to study the early events during cold ischemia/reperfusion (I/R) injury. This model, using the cuff technique, does not require extensive training and is relatively easy to be established. The model can induce reproducible degrees of pulmonary graft injury including impaired gas exchange, proinflammatory cytokine upregulation, or inflammatory infiltrates, depending on the preservation time. The results are consistent with the previous clinical evidence, thus suggesting that this model is a valid and reliable animal model of cold I/R injury.
KW - Cold preservation
KW - Experimental animal model
KW - Ischemia/reperfusion
KW - Lung transplantation
UR - http://www.scopus.com/inward/record.url?scp=67651165272&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67651165272&partnerID=8YFLogxK
U2 - 10.1007/s00595-008-3929-x
DO - 10.1007/s00595-008-3929-x
M3 - Article
VL - 39
SP - 641
EP - 645
JO - Japanese Journal of Surgery
JF - Japanese Journal of Surgery
SN - 0941-1291
IS - 7
ER -