Experimental orthotopic lung transplantation model in rats with cold storage

Ryujiro Sugimoto; Atsunori Nakao; Itaru Nagahiro; Junichi Kohmoto; Seiichiro Sugimoto; Mikio Okazaki; Masaomi Yamane; Hidetoshi Inokawa; Takahiro Oto; Kazunori Tahara; Jianghua Zhan; Yoshifumi Sano; Kenneth R. McCurry

doi:10.1007/s00595-008-3929-x

Experimental orthotopic lung transplantation model in rats with cold storage

Ryujiro Sugimoto, Atsunori Nakao, Itaru Nagahiro, Junichi Kohmoto, Seiichiro Sugimoto, Mikio Okazaki, Masaomi Yamane, Hidetoshi Inokawa, Takahiro Oto, Kazunori Tahara, Jianghua Zhan, Yoshifumi Sano, Kenneth R. McCurry

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

This report describes a new experimental procedure, a rat unilateral, orthotopic lung transplantation with cold storage, and evaluates its relevancy and reliability to study the early events during cold ischemia/reperfusion (I/R) injury. This model, using the cuff technique, does not require extensive training and is relatively easy to be established. The model can induce reproducible degrees of pulmonary graft injury including impaired gas exchange, proinflammatory cytokine upregulation, or inflammatory infiltrates, depending on the preservation time. The results are consistent with the previous clinical evidence, thus suggesting that this model is a valid and reliable animal model of cold I/R injury.

Original language	English
Pages (from-to)	641-645
Number of pages	5
Journal	Surgery today
Volume	39
Issue number	7
DOIs	https://doi.org/10.1007/s00595-008-3929-x
Publication status	Published - Jul 2009

Keywords

Cold preservation
Experimental animal model
Ischemia/reperfusion
Lung transplantation

ASJC Scopus subject areas

Surgery

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10.1007/s00595-008-3929-x

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title = "Experimental orthotopic lung transplantation model in rats with cold storage",

abstract = "This report describes a new experimental procedure, a rat unilateral, orthotopic lung transplantation with cold storage, and evaluates its relevancy and reliability to study the early events during cold ischemia/reperfusion (I/R) injury. This model, using the cuff technique, does not require extensive training and is relatively easy to be established. The model can induce reproducible degrees of pulmonary graft injury including impaired gas exchange, proinflammatory cytokine upregulation, or inflammatory infiltrates, depending on the preservation time. The results are consistent with the previous clinical evidence, thus suggesting that this model is a valid and reliable animal model of cold I/R injury.",

keywords = "Cold preservation, Experimental animal model, Ischemia/reperfusion, Lung transplantation",

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AU - Sugimoto, Ryujiro

AU - Nakao, Atsunori

AU - Nagahiro, Itaru

AU - Kohmoto, Junichi

AU - Sugimoto, Seiichiro

AU - Okazaki, Mikio

AU - Yamane, Masaomi

AU - Inokawa, Hidetoshi

AU - Oto, Takahiro

AU - Tahara, Kazunori

AU - Zhan, Jianghua

AU - Sano, Yoshifumi

AU - McCurry, Kenneth R.

PY - 2009/7

Y1 - 2009/7

N2 - This report describes a new experimental procedure, a rat unilateral, orthotopic lung transplantation with cold storage, and evaluates its relevancy and reliability to study the early events during cold ischemia/reperfusion (I/R) injury. This model, using the cuff technique, does not require extensive training and is relatively easy to be established. The model can induce reproducible degrees of pulmonary graft injury including impaired gas exchange, proinflammatory cytokine upregulation, or inflammatory infiltrates, depending on the preservation time. The results are consistent with the previous clinical evidence, thus suggesting that this model is a valid and reliable animal model of cold I/R injury.

AB - This report describes a new experimental procedure, a rat unilateral, orthotopic lung transplantation with cold storage, and evaluates its relevancy and reliability to study the early events during cold ischemia/reperfusion (I/R) injury. This model, using the cuff technique, does not require extensive training and is relatively easy to be established. The model can induce reproducible degrees of pulmonary graft injury including impaired gas exchange, proinflammatory cytokine upregulation, or inflammatory infiltrates, depending on the preservation time. The results are consistent with the previous clinical evidence, thus suggesting that this model is a valid and reliable animal model of cold I/R injury.

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KW - Experimental animal model

KW - Ischemia/reperfusion

KW - Lung transplantation

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Experimental orthotopic lung transplantation model in rats with cold storage

Abstract

Keywords

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