Experimental orthotopic lung transplantation model in rats with cold storage

Ryujiro Sugimoto, Atsunori Nakao, Itaru Nagahiro, Junichi Kohmoto, Seiichiro Sugimoto, Mikio Okazaki, Masaomi Yamane, Hidetoshi Inokawa, Takahiro Oto, Kazunori Tahara, Jianghua Zhan, Yoshifumi Sano, Kenneth R. McCurry

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This report describes a new experimental procedure, a rat unilateral, orthotopic lung transplantation with cold storage, and evaluates its relevancy and reliability to study the early events during cold ischemia/reperfusion (I/R) injury. This model, using the cuff technique, does not require extensive training and is relatively easy to be established. The model can induce reproducible degrees of pulmonary graft injury including impaired gas exchange, proinflammatory cytokine upregulation, or inflammatory infiltrates, depending on the preservation time. The results are consistent with the previous clinical evidence, thus suggesting that this model is a valid and reliable animal model of cold I/R injury.

Original languageEnglish
Pages (from-to)641-645
Number of pages5
JournalSurgery today
Volume39
Issue number7
DOIs
Publication statusPublished - Jul 1 2009

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Keywords

  • Cold preservation
  • Experimental animal model
  • Ischemia/reperfusion
  • Lung transplantation

ASJC Scopus subject areas

  • Surgery

Cite this

Sugimoto, R., Nakao, A., Nagahiro, I., Kohmoto, J., Sugimoto, S., Okazaki, M., Yamane, M., Inokawa, H., Oto, T., Tahara, K., Zhan, J., Sano, Y., & McCurry, K. R. (2009). Experimental orthotopic lung transplantation model in rats with cold storage. Surgery today, 39(7), 641-645. https://doi.org/10.1007/s00595-008-3929-x