Experimental Infection of Rabbits with Human T-Cell Leukemia Virus Type I

Tadaatsu Akaui, Shoki Yano, Isao Takeda, Isao Miyoshi, Takashi Oka, Yuji Ohtsuki

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38 Citations (Scopus)


Rabbits were successfully infected with human T-cell leukemia virus type I (HTLV-I) and produced antibodies to adult T-cell leukemia-associated antigens (ATLA) on intravenous inoculation of the HTLV-producing human cord T-cell line MT-2, or autologous cell lines established by cocultivation with MT-2 cells. Lymphocytes taken from the rabbits between 4 and 14 days after the inoculation of MT-2 cells, but not lymphocytes obtained in earlier or later periods, could be immortalized in vitro and expressed ATLA and type C virus particles. However, lymphocytes harvested during an early culture period (5 to 8 days) were found to be negative for ATLA. The transformed cells had the karyotype of a normal male rabbit. Two rabbits inoculated with the autologous HTLV-producing cell lines did not allow their growth in vivo, but some peripheral blood lymphocytes could be immortalized in vitro. One of these transformed cell lines was examined for the integration of HTLV-I provirus genome. The transformed cells were found to contain the provirus genome and also to be monoclonal with respect to the integration site of the provirus genome, unlike the inoculated cells.

Original languageEnglish
Pages (from-to)86-94
Number of pages9
JournalJapanese Journal of Cancer Research GANN
Issue number2
Publication statusPublished - 1985
Externally publishedYes


  • Experimental infection
  • Human T-cell leukemia virus
  • Rabbit lymphocytes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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