Experimental allergic conjunctivitis in guinea pigs induced by Japanese cedar pollen

Miho Takada, Tamao Yamada, Hiroto Nakahara, Yukio Sugimoto, Keiji Izushi, Chiaki Kamei

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We report a new experimental allergic conjunctivitis with Japanese cedar pollen as antigen in guinea pigs, and the immunological characteristics of this model were also elucidated. Allergic conjunctivitis was developed by immunization in guinea pigs with a mixture containing Japanese cedar pollen and killed Bordetella pertussis. When local application of Japanese cedar pollen suspension 14 d after systemic immunization was performed every 3 d, remarkable conjunctivitis was observed from 20 to 35d. Increase in vascular permeability and decrease in histamine contents of the conjunctiva were also observed after local application of antigen. Passive cutaneous anaphylactic (PCA) reactions revealed that both IgG- and IgE-rich antibodies were produced in this model. Chlorpheniramine, ketotifen and levocabastine were effective in inhibiting cedar pollen-induced conjunctivitis. Although a high concentration was needed, tranilast and amlexanox also showed significant inhibition of conjunctivitis induced by cedar pollen.

Original languageEnglish
Pages (from-to)566-569
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume23
Issue number5
Publication statusPublished - May 2000

Fingerprint

Cryptomeria
Allergic Conjunctivitis
Pollen
Guinea Pigs
Conjunctivitis
levocabastine
Immunization
Immunological Models
Ketotifen
Chlorpheniramine
Antigens
Bordetella pertussis
Conjunctiva
Capillary Permeability
Anaphylaxis
Immunoglobulin E
Histamine
Suspensions
Immunoglobulin G
Skin

Keywords

  • Allergic conjunctivitis
  • Amlexanox
  • Chlorpheniramine
  • IgG antibody
  • Japanese cedar pollen
  • Ketotifen

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Experimental allergic conjunctivitis in guinea pigs induced by Japanese cedar pollen. / Takada, Miho; Yamada, Tamao; Nakahara, Hiroto; Sugimoto, Yukio; Izushi, Keiji; Kamei, Chiaki.

In: Biological and Pharmaceutical Bulletin, Vol. 23, No. 5, 05.2000, p. 566-569.

Research output: Contribution to journalArticle

Takada, M, Yamada, T, Nakahara, H, Sugimoto, Y, Izushi, K & Kamei, C 2000, 'Experimental allergic conjunctivitis in guinea pigs induced by Japanese cedar pollen', Biological and Pharmaceutical Bulletin, vol. 23, no. 5, pp. 566-569.
Takada, Miho ; Yamada, Tamao ; Nakahara, Hiroto ; Sugimoto, Yukio ; Izushi, Keiji ; Kamei, Chiaki. / Experimental allergic conjunctivitis in guinea pigs induced by Japanese cedar pollen. In: Biological and Pharmaceutical Bulletin. 2000 ; Vol. 23, No. 5. pp. 566-569.
@article{acda2addeefb4299ab5fd22b1baf937c,
title = "Experimental allergic conjunctivitis in guinea pigs induced by Japanese cedar pollen",
abstract = "We report a new experimental allergic conjunctivitis with Japanese cedar pollen as antigen in guinea pigs, and the immunological characteristics of this model were also elucidated. Allergic conjunctivitis was developed by immunization in guinea pigs with a mixture containing Japanese cedar pollen and killed Bordetella pertussis. When local application of Japanese cedar pollen suspension 14 d after systemic immunization was performed every 3 d, remarkable conjunctivitis was observed from 20 to 35d. Increase in vascular permeability and decrease in histamine contents of the conjunctiva were also observed after local application of antigen. Passive cutaneous anaphylactic (PCA) reactions revealed that both IgG- and IgE-rich antibodies were produced in this model. Chlorpheniramine, ketotifen and levocabastine were effective in inhibiting cedar pollen-induced conjunctivitis. Although a high concentration was needed, tranilast and amlexanox also showed significant inhibition of conjunctivitis induced by cedar pollen.",
keywords = "Allergic conjunctivitis, Amlexanox, Chlorpheniramine, IgG antibody, Japanese cedar pollen, Ketotifen",
author = "Miho Takada and Tamao Yamada and Hiroto Nakahara and Yukio Sugimoto and Keiji Izushi and Chiaki Kamei",
year = "2000",
month = "5",
language = "English",
volume = "23",
pages = "566--569",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "5",

}

TY - JOUR

T1 - Experimental allergic conjunctivitis in guinea pigs induced by Japanese cedar pollen

AU - Takada, Miho

AU - Yamada, Tamao

AU - Nakahara, Hiroto

AU - Sugimoto, Yukio

AU - Izushi, Keiji

AU - Kamei, Chiaki

PY - 2000/5

Y1 - 2000/5

N2 - We report a new experimental allergic conjunctivitis with Japanese cedar pollen as antigen in guinea pigs, and the immunological characteristics of this model were also elucidated. Allergic conjunctivitis was developed by immunization in guinea pigs with a mixture containing Japanese cedar pollen and killed Bordetella pertussis. When local application of Japanese cedar pollen suspension 14 d after systemic immunization was performed every 3 d, remarkable conjunctivitis was observed from 20 to 35d. Increase in vascular permeability and decrease in histamine contents of the conjunctiva were also observed after local application of antigen. Passive cutaneous anaphylactic (PCA) reactions revealed that both IgG- and IgE-rich antibodies were produced in this model. Chlorpheniramine, ketotifen and levocabastine were effective in inhibiting cedar pollen-induced conjunctivitis. Although a high concentration was needed, tranilast and amlexanox also showed significant inhibition of conjunctivitis induced by cedar pollen.

AB - We report a new experimental allergic conjunctivitis with Japanese cedar pollen as antigen in guinea pigs, and the immunological characteristics of this model were also elucidated. Allergic conjunctivitis was developed by immunization in guinea pigs with a mixture containing Japanese cedar pollen and killed Bordetella pertussis. When local application of Japanese cedar pollen suspension 14 d after systemic immunization was performed every 3 d, remarkable conjunctivitis was observed from 20 to 35d. Increase in vascular permeability and decrease in histamine contents of the conjunctiva were also observed after local application of antigen. Passive cutaneous anaphylactic (PCA) reactions revealed that both IgG- and IgE-rich antibodies were produced in this model. Chlorpheniramine, ketotifen and levocabastine were effective in inhibiting cedar pollen-induced conjunctivitis. Although a high concentration was needed, tranilast and amlexanox also showed significant inhibition of conjunctivitis induced by cedar pollen.

KW - Allergic conjunctivitis

KW - Amlexanox

KW - Chlorpheniramine

KW - IgG antibody

KW - Japanese cedar pollen

KW - Ketotifen

UR - http://www.scopus.com/inward/record.url?scp=0034032412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034032412&partnerID=8YFLogxK

M3 - Article

C2 - 10823665

AN - SCOPUS:0034032412

VL - 23

SP - 566

EP - 569

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 5

ER -