TY - GEN
T1 - Expansion of protein biosynthesis system including nonnatural amino acids
AU - Ohtsuki, Takashi
AU - Doi, Yoshio
AU - Manabe, Taishi
AU - Sisido, Masahiko
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Nonnatural amino acids, such as fluorescent amino acids, can be introduced into proteins by using an expanded protein biosynthesis system. This approach requires orthogonal tRNAs that deliver nonnatural amino acids to a ribosome and expanded codon/anticodon pairs, like four-base codon/anticodon pairs. However, in this system, some of nonnatural amino acids are not efficiently incorporated into proteins. To expand the availability of the nonnatural mugatenesis, translation factors and tRNAs must be improved. In this study, we designed and synthesized an elongation factor Tu (EF-Tu) mutant that efficiently binds to tRNAs charged with nonnatural amino acids. We also devised new tRNAs that decode four-base codons and efficiently deliver nonnatural amino acids into ribosome.
AB - Nonnatural amino acids, such as fluorescent amino acids, can be introduced into proteins by using an expanded protein biosynthesis system. This approach requires orthogonal tRNAs that deliver nonnatural amino acids to a ribosome and expanded codon/anticodon pairs, like four-base codon/anticodon pairs. However, in this system, some of nonnatural amino acids are not efficiently incorporated into proteins. To expand the availability of the nonnatural mugatenesis, translation factors and tRNAs must be improved. In this study, we designed and synthesized an elongation factor Tu (EF-Tu) mutant that efficiently binds to tRNAs charged with nonnatural amino acids. We also devised new tRNAs that decode four-base codons and efficiently deliver nonnatural amino acids into ribosome.
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U2 - 10.1109/MHS.2006.320251
DO - 10.1109/MHS.2006.320251
M3 - Conference contribution
AN - SCOPUS:50449086735
SN - 1424407176
SN - 9781424407170
T3 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
BT - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
T2 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
Y2 - 5 November 2006 through 8 November 2006
ER -