Expansion of protein biosynthesis system including nonnatural amino acids

Takashi Ohtsuki; Yoshio Doi; Taishi Manabe; Masahiko Sisido

doi:10.1109/MHS.2006.320251

Expansion of protein biosynthesis system including nonnatural amino acids

Takashi Ohtsuki, Yoshio Doi, Taishi Manabe, Masahiko Sisido

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Nonnatural amino acids, such as fluorescent amino acids, can be introduced into proteins by using an expanded protein biosynthesis system. This approach requires orthogonal tRNAs that deliver nonnatural amino acids to a ribosome and expanded codon/anticodon pairs, like four-base codon/anticodon pairs. However, in this system, some of nonnatural amino acids are not efficiently incorporated into proteins. To expand the availability of the nonnatural mugatenesis, translation factors and tRNAs must be improved. In this study, we designed and synthesized an elongation factor Tu (EF-Tu) mutant that efficiently binds to tRNAs charged with nonnatural amino acids. We also devised new tRNAs that decode four-base codons and efficiently deliver nonnatural amino acids into ribosome.

Original language	English
Title of host publication	2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
DOIs	https://doi.org/10.1109/MHS.2006.320251
Publication status	Published - Dec 1 2006
Event	2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS - Nagoya, Japan Duration: Nov 5 2006 → Nov 8 2006

Publication series

Name	2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

Other

Other	2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
Country/Territory	Japan
City	Nagoya
Period	11/5/06 → 11/8/06

ASJC Scopus subject areas

Computational Mechanics
Control and Systems Engineering
Human Factors and Ergonomics

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10.1109/MHS.2006.320251

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Expansion of protein biosynthesis system including nonnatural amino acids. / Ohtsuki, Takashi; Doi, Yoshio; Manabe, Taishi et al.

2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS. 2006. 4110406 (2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Ohtsuki, T, Doi, Y, Manabe, T & Sisido, M 2006, Expansion of protein biosynthesis system including nonnatural amino acids. in 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS., 4110406, 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS, 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS, Nagoya, Japan, 11/5/06. https://doi.org/10.1109/MHS.2006.320251

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abstract = "Nonnatural amino acids, such as fluorescent amino acids, can be introduced into proteins by using an expanded protein biosynthesis system. This approach requires orthogonal tRNAs that deliver nonnatural amino acids to a ribosome and expanded codon/anticodon pairs, like four-base codon/anticodon pairs. However, in this system, some of nonnatural amino acids are not efficiently incorporated into proteins. To expand the availability of the nonnatural mugatenesis, translation factors and tRNAs must be improved. In this study, we designed and synthesized an elongation factor Tu (EF-Tu) mutant that efficiently binds to tRNAs charged with nonnatural amino acids. We also devised new tRNAs that decode four-base codons and efficiently deliver nonnatural amino acids into ribosome.",

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AB - Nonnatural amino acids, such as fluorescent amino acids, can be introduced into proteins by using an expanded protein biosynthesis system. This approach requires orthogonal tRNAs that deliver nonnatural amino acids to a ribosome and expanded codon/anticodon pairs, like four-base codon/anticodon pairs. However, in this system, some of nonnatural amino acids are not efficiently incorporated into proteins. To expand the availability of the nonnatural mugatenesis, translation factors and tRNAs must be improved. In this study, we designed and synthesized an elongation factor Tu (EF-Tu) mutant that efficiently binds to tRNAs charged with nonnatural amino acids. We also devised new tRNAs that decode four-base codons and efficiently deliver nonnatural amino acids into ribosome.

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Expansion of protein biosynthesis system including nonnatural amino acids

Abstract

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Other

ASJC Scopus subject areas

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