Expansion of primary human hepatocyte populations by retroviral gene transfer and adenovirus-mediated CRE/LOXP site-specific recombination

N. Kobayashi, H. Noguchi, Toshiyoshi Fujiwara, N. Tanaka, K. A. Westerman, P. Leboulch

Research output: Contribution to journalArticle

Abstract

Human primary hepatocytes are ideal for bioartificial liver (BAL), however, the shortage of human livers available for hepatocyte isolation severely limits the use of this modality. To resolve this issue, adult human hepatocytes were immortalized with a retrovector containing the genes encoding Simian virus 40 T antigen flanked by a pair of Ioxp recombination target that was subsequently excised by Cre recombinase. Based on the observations, a reversible immortalization system in primary adult human hepatocytes was established.

Original languageEnglish
Pages (from-to)229
Number of pages1
JournalASAIO Journal
Volume46
Issue number2
Publication statusPublished - Mar 2000

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Gene transfer
Adenoviridae
Genetic Recombination
Hepatocytes
Liver
Population
Genes
Artificial Liver
Gene encoding
Simian virus 40
Viral Tumor Antigens
Antigens
Viruses

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Expansion of primary human hepatocyte populations by retroviral gene transfer and adenovirus-mediated CRE/LOXP site-specific recombination. / Kobayashi, N.; Noguchi, H.; Fujiwara, Toshiyoshi; Tanaka, N.; Westerman, K. A.; Leboulch, P.

In: ASAIO Journal, Vol. 46, No. 2, 03.2000, p. 229.

Research output: Contribution to journalArticle

Kobayashi, N. ; Noguchi, H. ; Fujiwara, Toshiyoshi ; Tanaka, N. ; Westerman, K. A. ; Leboulch, P. / Expansion of primary human hepatocyte populations by retroviral gene transfer and adenovirus-mediated CRE/LOXP site-specific recombination. In: ASAIO Journal. 2000 ; Vol. 46, No. 2. pp. 229.
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