Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in vitro

Ayako Aoki-Yoshida, Shinichi Saito, Takeshi Tsuruta, Arisa Ohsumi, Hinako Tsunoda, Kei Sonoyama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Orally administered Lactobacillus strains, including L. plantarum No.14 and L. rhamnosus GG, reportedly reduce inflammatory cytokine production in mice. The present study tested our idea that circulating exosomes mediate the action of Lactobacillus strains. The lipopolysaccharide-induced production of TNF-α and IL-6 in vitro was attenuated in peritoneal exudate cells (PECs) isolated from C57BL/6N mice that had been fed L. plantarum No.14. When PECs were cultured for 24 h with exosomes isolated from the serum of mice fed L. plantarum No.14 or L. rhamnosus GG, accumulation of both TNF-α and of the corresponding mRNA was lowered. Growth in the presence of these exosomes also decreased the production of TNF-α and IL-6 by the murine macrophage cell line RAW264.7. In contrast, supplementation with exosome-depleted serum of mice fed L. plantarum No.14 or L. rhamnosus GG failed to affect the production of TNF-α and IL-6 by RAW264.7 cells. When PECs and RAW264.7 cells were cultured for 24 h with PKH67-labeled exosomes isolated from murine serum, fluorescent signal was observed inside the cells, suggesting that these cells incorporate serum exosomes. We propose that the anti-inflammatory activity of orally administered L. plantarum No.14 and L. rhamnosus GG is mediated, at least in part, by circulating exosomes.

Original languageEnglish
Pages (from-to)248-254
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume489
Issue number2
DOIs
Publication statusPublished - Jul 22 2017

Fingerprint

Exosomes
Macrophages
Lactobacillus
Cytokines
Interleukin-6
Serum
Exudates and Transudates
Cultured Cells
Lipopolysaccharides
Anti-Inflammatory Agents
Cells
Messenger RNA
Inbred C57BL Mouse
Cell Line
Growth

Keywords

  • Exosome
  • IL-6
  • Inflammation
  • Probiotics
  • TNF-α

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in vitro. / Aoki-Yoshida, Ayako; Saito, Shinichi; Tsuruta, Takeshi; Ohsumi, Arisa; Tsunoda, Hinako; Sonoyama, Kei.

In: Biochemical and Biophysical Research Communications, Vol. 489, No. 2, 22.07.2017, p. 248-254.

Research output: Contribution to journalArticle

Aoki-Yoshida, Ayako ; Saito, Shinichi ; Tsuruta, Takeshi ; Ohsumi, Arisa ; Tsunoda, Hinako ; Sonoyama, Kei. / Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in vitro. In: Biochemical and Biophysical Research Communications. 2017 ; Vol. 489, No. 2. pp. 248-254.
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