Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities

Md Anamul Hoque, Eiji Okuma, Mst Nasrin Akhter Banu, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata

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185 Citations (Scopus)

Abstract

Proline and betaine accumulate in plant cells under environmental stresses including salt stress. Here, we investigated effects of proline and betaine on the growth and activities of antioxidant enzymes in tobacco Bright Yellow-2 (BY-2) culture cells in suspension under salt stress. Both proline and betaine mitigated the inhibition of growth of BY-2 cells under salt stress and the mitigating effect of proline was more than that of betaine. Salt stress significantly decreased the activities of superoxide dismutase (SOD), catalase and peroxidase in BY-2 cells. Exogenous application of proline or betaine alleviated the reduction in catalase and peroxidase activities but not SOD activity under salt stress. In addition, proline was found to be effective in alleviating the inhibition of salt stress-induced catalase and peroxidase activities in BY-2 cells. Neither proline nor betaine directly scavenged superoxide (O2-) or hydrogen peroxide (H2O2). It is concluded that exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine because of its superior ability to increase the activities of antioxidant enzymes.

Original languageEnglish
Pages (from-to)553-561
Number of pages9
JournalJournal of Plant Physiology
Volume164
Issue number5
DOIs
Publication statusPublished - May 3 2007

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Keywords

  • Antioxidant enzymes
  • Betaine
  • Proline
  • Reactive oxygen species
  • Salt stress

ASJC Scopus subject areas

  • Plant Science

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