Exogenous proline enhances the sensitivity of Tobacco BY-2 cells to arsenate

Mst Nur E.Nazmun Nahar, Mohammad Muzahidul Islam, Md Anamul Hoque, Anna Yonezawa, Md Yeasin Prodhan, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Arsenic causes physiological and structural disorders in plants. Proline is accumulated as a compatible solute in plants under various stress conditions and mitigates stresses. Here, we investigated the effects of exogenous proline on tobacco Bright Yellow-2 (BY-2) cultured cells under AsO4 - stress. Arsenate did not inhibit BY-2 cell growth at 40 and 50 μM but did it at 60 μM. Proline at 0.5 to 10 mM did not affect the cell growth but delayed it at 20 mM. At 40 μM AsO4 -, neither 0.5 mM nor 1 mM proline affected the cell growth but 10 mM proline inhibited it. In the presence of AsO4 -, 10 mM proline increased the number of Evans Blue-stained (dead) cells and decreased the number of total cells. Together, our results suggest that exogenous proline does not alleviate arsenate toxicity but enhances the sensitivity of BY-2 cells to arsenate.

Original languageEnglish
Pages (from-to)1726-1731
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume81
Issue number9
DOIs
Publication statusPublished - 2017

Keywords

  • Arsenic
  • Cell death
  • Cell growth
  • Cell number
  • Proline

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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