Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells

Mohammad Muzahidul Islam, Md Anamul Hoque, Eiji Okuma, Mst Nasrin Akhter Banu, Yasuaki Shimoishi, Yoshimasa Nakamura, Yoshiyuki Murata

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Environmental stress, including heavy metal stress, can cause oxidative damage to plants. Up-regulation of the antioxidant defense system induced by proline and glycinebetaine (betaine) alleviates the damaging effects of oxidative stress in plants. Here, we investigated the protective effects of exogenously applied proline and betaine on growth, accumulation of proline and betaine, lipid peroxidation and activity of antioxidant enzymes in cultured tobacco Bright Yellow-2 (BY-2) cells exposed to cadmium (Cd) stress. Cadmium stress (at 100 μM Cd) caused a significant inhibition of the growth of BY-2 cells, and both proline and betaine significantly mitigated this inhibition. In addition, the mitigating effect of proline was more pronounced than that of betaine. Cadmium stress leads to an accumulation of Cd and endogenous proline in cultured cells, increased lipid peroxidation and peroxidase (POX) activity, and decreased activity of superoxide dismutase (SOD) and catalase (CAT). Exogenous application of proline resulted in a decrease in lipid peroxidation and an increase in SOD and CAT activities without reducing Cd contents under Cd stress, while application of betaine resulted in a decrease in lipid peroxidation and an increase in CAT activity with reducing Cd accumulation. Furthermore, exogenous proline and betaine intensified the accumulation of proline and betaine in Cd-stressed BY-2 cells, respectively. The present study suggests that proline and betaine confer tolerance to Cd stress in tobacco BY-2 cells by different mechanisms.

Original languageEnglish
Pages (from-to)1587-1597
Number of pages11
JournalJournal of Plant Physiology
Volume166
Issue number15
DOIs
Publication statusPublished - Oct 15 2009

Fingerprint

betaine
Cadmium
Proline
Tobacco
proline
cadmium
Cultured Cells
tobacco
Antioxidants
enzyme activity
antioxidants
Enzymes
Lipid Peroxidation
cells
lipid peroxidation
Catalase
Betaine
catalase
Superoxide Dismutase
superoxide dismutase

Keywords

  • Antioxidant enzymes
  • Cadmium
  • Glycinebetaine
  • Proline
  • Reactive oxygen species

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells. / Islam, Mohammad Muzahidul; Hoque, Md Anamul; Okuma, Eiji; Banu, Mst Nasrin Akhter; Shimoishi, Yasuaki; Nakamura, Yoshimasa; Murata, Yoshiyuki.

In: Journal of Plant Physiology, Vol. 166, No. 15, 15.10.2009, p. 1587-1597.

Research output: Contribution to journalArticle

Islam, Mohammad Muzahidul ; Hoque, Md Anamul ; Okuma, Eiji ; Banu, Mst Nasrin Akhter ; Shimoishi, Yasuaki ; Nakamura, Yoshimasa ; Murata, Yoshiyuki. / Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells. In: Journal of Plant Physiology. 2009 ; Vol. 166, No. 15. pp. 1587-1597.
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