Existence of an iron-oxidizing Bacterium acidithiobacillus ferrooxidans resistant to organomercurial compounds

Fumiaki Takeuchi, Atsunori Negishi, Sosaku Nakamura, Tadayoshi Kanao, Kazuo Kamimura, Tsuyoshi Sugio

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Acidithiobacillus ferrooxidans MON-1 which is highly resistant to Hg 2+ could grow in a ferrous sulfate medium (pH 2.5) with 0.1 μM p-chloromercuribenzoic acid (PCMB) with a lag time of 2 d. In contrast, A. ferrooxidans AP19-3 which is sensitive to Hg2+ did not grow in the medium. Nine strains of A. ferrooxidans, including seven strains of the American Type Culture Collection grew in the medium with a lag time ranging from 5 to 12 d. The resting cells of MON-1, which has NADPH-dependent mercuric reductase activity, could volatilize Hg0 when incubated in acidic water (pH3.0) containing 0.1 μM PCMB. However, the resting cells of AP19-3, which has a similar level of NADPH-dependent mercuric reductase activity compared with MON-1, did not volatilize Hg0 from the reaction mixture with 0.1 μM -e;e;PCMB. The activity level of the 11 strains of A. ferrooxidans to volatilize Hg0 from PCMB corresponded well with the level of growth inhibition by PCMB observed in the growth experiments. The resting cells of MON-1 volatilized Hg0 from phenylmercury acetate (PMA) and methylmercury chloride (MMC) as well as PCMB. The cytosol prepared from MON-1 could volatilize Hg0 from PCMB (0.015 nmol mg-11), PMA (0.33 nmol rag-1 h-1) and MMC (0.005 nmol mg-1 h-1) in the presence of NADPH and β-mercaptoethanol.

Original languageEnglish
Pages (from-to)586-591
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume99
Issue number6
DOIs
Publication statusPublished - 2005

Fingerprint

Acidithiobacillus
p-Chloromercuribenzoic Acid
Bacteria
Iron
Acids
NADP
ferrous sulfate
Acetates
Mercaptoethanol
Growth
Cytosol
Water

Keywords

  • Acidithiobacillus ferrooxidans
  • Iron-oxidizing bacterium
  • Mercuric reductase
  • Organomercurial compounds

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Existence of an iron-oxidizing Bacterium acidithiobacillus ferrooxidans resistant to organomercurial compounds. / Takeuchi, Fumiaki; Negishi, Atsunori; Nakamura, Sosaku; Kanao, Tadayoshi; Kamimura, Kazuo; Sugio, Tsuyoshi.

In: Journal of Bioscience and Bioengineering, Vol. 99, No. 6, 2005, p. 586-591.

Research output: Contribution to journalArticle

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abstract = "Acidithiobacillus ferrooxidans MON-1 which is highly resistant to Hg 2+ could grow in a ferrous sulfate medium (pH 2.5) with 0.1 μM p-chloromercuribenzoic acid (PCMB) with a lag time of 2 d. In contrast, A. ferrooxidans AP19-3 which is sensitive to Hg2+ did not grow in the medium. Nine strains of A. ferrooxidans, including seven strains of the American Type Culture Collection grew in the medium with a lag time ranging from 5 to 12 d. The resting cells of MON-1, which has NADPH-dependent mercuric reductase activity, could volatilize Hg0 when incubated in acidic water (pH3.0) containing 0.1 μM PCMB. However, the resting cells of AP19-3, which has a similar level of NADPH-dependent mercuric reductase activity compared with MON-1, did not volatilize Hg0 from the reaction mixture with 0.1 μM -e;e;PCMB. The activity level of the 11 strains of A. ferrooxidans to volatilize Hg0 from PCMB corresponded well with the level of growth inhibition by PCMB observed in the growth experiments. The resting cells of MON-1 volatilized Hg0 from phenylmercury acetate (PMA) and methylmercury chloride (MMC) as well as PCMB. The cytosol prepared from MON-1 could volatilize Hg0 from PCMB (0.015 nmol mg-11), PMA (0.33 nmol rag-1 h-1) and MMC (0.005 nmol mg-1 h-1) in the presence of NADPH and β-mercaptoethanol.",
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