Existence of aa3-type ubiquinol oxidase as a terminal oxidase in sulfite oxidation of Acidithiobacillus thiooxidans

Tsuyoshi Sugio, Tomohiro Hisazumi, Tadayoshi Kanao, Kazuo Kamimura, Fumiaki Takeuchi, Atsunori Negishi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

It was found that Acidithiobacillus thiooxidans has sulfite:ubiquinone oxidoreductase and ubiquinol oxidase activities in the cells. Ubiquinol oxidase was purified from plasma membranes of strain NB1-3 in a nearly homogeneous state. A purified enzyme showed absorption peaks at 419 and 595 nm in the oxidized form and at 442 and 605 nm in the reduced form. Pyridine ferrohaemochrome prepared from the enzyme showed an α-peak characteristic of haem a at 587 nm, indicating that the enzyme contains haem a as a component. The CO difference spectrum of ubiquinol oxidase showed two peaks at 428 nm and 595 nm, and a trough at 446 nm, suggesting the existence of an aa 3-type cytochrome in the enzyme. Ubiquinol oxidase was composed of three subunits with apparent molecular masses of 57 kDa, 34 kDa, and 23 kDa. The optimum pH and temperature for ubiquinol oxidation were pH 6.0 and 30°C. The activity was completely inhibited by sodium cyanide at 1.0 mM. In contrast, the activity was inhibited weakly by antimycin A1 and myxothiazol, which are inhibitors of mitochondrial bc1 complex. Quinone analog 2-heptyl-4-hydoroxyquinoline N-oxide (HOQ-NO) strongly inhibited ubiquinol oxidase activity. Nickel and tungstate (0.1 mM), which are used as a bacteriostatic agent for A. thiooxidans-dependent concrete corrosion, inhibited ubiquinol oxidase activity 100 and 70% respectively.

Original languageEnglish
Pages (from-to)1584-1591
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number7
DOIs
Publication statusPublished - 2006

Fingerprint

Acidithiobacillus thiooxidans
sulfite oxidase
Sulfites
Oxidoreductases
oxidation
Oxidation
enzymes
Enzymes
Heme
ubiquinones
pyridines
sulfites
corrosion
oxidoreductases
cyanides
cytochromes
quinones
nickel
Sodium Cyanide
Antimycin A

Keywords

  • Acidithiobacillus thiooxidans
  • Concrete corrosion
  • Sulfite oxidation
  • Terminal oxidase
  • Ubiquinol oxidase

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Existence of aa3-type ubiquinol oxidase as a terminal oxidase in sulfite oxidation of Acidithiobacillus thiooxidans. / Sugio, Tsuyoshi; Hisazumi, Tomohiro; Kanao, Tadayoshi; Kamimura, Kazuo; Takeuchi, Fumiaki; Negishi, Atsunori.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 7, 2006, p. 1584-1591.

Research output: Contribution to journalArticle

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