Existence of a novel hemagglutinin having no protease activity in Vibrio mimicus

M. Alam, Shin-ichi Miyoshi, I. Maruo, C. Ogawa, S. Shinoda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The protease elaborated by Vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known HA/protease. A non-protease hemagglutinin (HA) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of V. mimicus. This HA (V. mimicus HA: VMHA) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocytes from chicken, bovine, horse and sheep. Simple sugars, metal ions and chelating agents failed to inhibit the activity of VMHA. The activity of VMHA was found to be sensitive to digestion by proteolytic enzymes including HA/protease. These results provide evidence for the existence of novel HA other than HA/protease in V. mimicus.

Original languageEnglish
Pages (from-to)467-470
Number of pages4
JournalMicrobiology and Immunology
Volume38
Issue number6
Publication statusPublished - 1994

Fingerprint

Vibrio mimicus
Hemagglutinins
Peptide Hydrolases
Erythrocytes
Chickens
Rabbits
Chelating Agents
Horses
Digestion
Sheep
Guinea Pigs
Ions
Temperature
hemagglutinin-protease

Keywords

  • HA/protease
  • Hemagglutinin
  • Vibrio mimicus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology
  • Microbiology (medical)

Cite this

Existence of a novel hemagglutinin having no protease activity in Vibrio mimicus. / Alam, M.; Miyoshi, Shin-ichi; Maruo, I.; Ogawa, C.; Shinoda, S.

In: Microbiology and Immunology, Vol. 38, No. 6, 1994, p. 467-470.

Research output: Contribution to journalArticle

Alam, M, Miyoshi, S, Maruo, I, Ogawa, C & Shinoda, S 1994, 'Existence of a novel hemagglutinin having no protease activity in Vibrio mimicus', Microbiology and Immunology, vol. 38, no. 6, pp. 467-470.
Alam, M. ; Miyoshi, Shin-ichi ; Maruo, I. ; Ogawa, C. ; Shinoda, S. / Existence of a novel hemagglutinin having no protease activity in Vibrio mimicus. In: Microbiology and Immunology. 1994 ; Vol. 38, No. 6. pp. 467-470.
@article{f55e0f9a35f7476ea095ed9c685bb4b1,
title = "Existence of a novel hemagglutinin having no protease activity in Vibrio mimicus",
abstract = "The protease elaborated by Vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known HA/protease. A non-protease hemagglutinin (HA) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of V. mimicus. This HA (V. mimicus HA: VMHA) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocytes from chicken, bovine, horse and sheep. Simple sugars, metal ions and chelating agents failed to inhibit the activity of VMHA. The activity of VMHA was found to be sensitive to digestion by proteolytic enzymes including HA/protease. These results provide evidence for the existence of novel HA other than HA/protease in V. mimicus.",
keywords = "HA/protease, Hemagglutinin, Vibrio mimicus",
author = "M. Alam and Shin-ichi Miyoshi and I. Maruo and C. Ogawa and S. Shinoda",
year = "1994",
language = "English",
volume = "38",
pages = "467--470",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "6",

}

TY - JOUR

T1 - Existence of a novel hemagglutinin having no protease activity in Vibrio mimicus

AU - Alam, M.

AU - Miyoshi, Shin-ichi

AU - Maruo, I.

AU - Ogawa, C.

AU - Shinoda, S.

PY - 1994

Y1 - 1994

N2 - The protease elaborated by Vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known HA/protease. A non-protease hemagglutinin (HA) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of V. mimicus. This HA (V. mimicus HA: VMHA) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocytes from chicken, bovine, horse and sheep. Simple sugars, metal ions and chelating agents failed to inhibit the activity of VMHA. The activity of VMHA was found to be sensitive to digestion by proteolytic enzymes including HA/protease. These results provide evidence for the existence of novel HA other than HA/protease in V. mimicus.

AB - The protease elaborated by Vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known HA/protease. A non-protease hemagglutinin (HA) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of V. mimicus. This HA (V. mimicus HA: VMHA) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocytes from chicken, bovine, horse and sheep. Simple sugars, metal ions and chelating agents failed to inhibit the activity of VMHA. The activity of VMHA was found to be sensitive to digestion by proteolytic enzymes including HA/protease. These results provide evidence for the existence of novel HA other than HA/protease in V. mimicus.

KW - HA/protease

KW - Hemagglutinin

KW - Vibrio mimicus

UR - http://www.scopus.com/inward/record.url?scp=0027980593&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027980593&partnerID=8YFLogxK

M3 - Article

C2 - 7968677

AN - SCOPUS:0027980593

VL - 38

SP - 467

EP - 470

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 6

ER -