Exclusive localization of tandem repetitive sequences in sulbtelomeric heterochromatin regions of Leymus racemosus (Poaceae, Triticeae)

Masahiro Kishii, Kiyotaka Nagaki, Hisashi Tsujimoto, Tetsuo Sasakuma

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Two kinds of tandem repetitive sequences were isolated from Leymus racemosus (Lam.) Tzvelev. One of them was classified in the 350-bp family originally isolated from Secale. The other was a novel repetitive sequence family, named 'TaiI family', which consisted of a repeat unit of 570 bp. Fluorescence in-situ hybridization of the chromosomes of L. racemosus indicated that both families were located in subtelomeric heterochromatin and that the 350-bp family and TaiI family occupied different heterochromatin regions. In addition, even homologous chromosomes did not show the same patterns of TaiI and 350-bp families. The combination of these two families of repetitive sequences, together with Afa-family sequences and rDNAs, helps to identify the ten homologous chromosome pairs of L. racemosus. From these data, we proposed a karyotype of L. racemosus and compared it with other karyotypes already reported.

Original languageEnglish
Pages (from-to)519-529
Number of pages11
JournalChromosome Research
Volume7
Issue number7
DOIs
Publication statusPublished - Nov 30 1999
Externally publishedYes

Keywords

  • 350-bp family sequences
  • Afa-family sequences
  • Leymus
  • Subtelomeric heterochromatin
  • Triticeae

ASJC Scopus subject areas

  • Genetics

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