Abstract
Two kinds of tandem repetitive sequences were isolated from Leymus racemosus (Lam.) Tzvelev. One of them was classified in the 350-bp family originally isolated from Secale. The other was a novel repetitive sequence family, named 'TaiI family', which consisted of a repeat unit of 570 bp. Fluorescence in-situ hybridization of the chromosomes of L. racemosus indicated that both families were located in subtelomeric heterochromatin and that the 350-bp family and TaiI family occupied different heterochromatin regions. In addition, even homologous chromosomes did not show the same patterns of TaiI and 350-bp families. The combination of these two families of repetitive sequences, together with Afa-family sequences and rDNAs, helps to identify the ten homologous chromosome pairs of L. racemosus. From these data, we proposed a karyotype of L. racemosus and compared it with other karyotypes already reported.
| Original language | English |
|---|---|
| Pages (from-to) | 519-529 |
| Number of pages | 11 |
| Journal | Chromosome Research |
| Volume | 7 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |
Fingerprint
Keywords
- 350-bp family sequences
- Afa-family sequences
- Leymus
- Subtelomeric heterochromatin
- Triticeae
ASJC Scopus subject areas
- Genetics
Cite this
Exclusive localization of tandem repetitive sequences in sulbtelomeric heterochromatin regions of Leymus racemosus (Poaceae, Triticeae). / Kishii, Masahiro; Nagaki, Kiyotaka; Tsujimoto, Hisashi; Sasakuma, Tetsuo.
In: Chromosome Research, Vol. 7, No. 7, 1999, p. 519-529.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Exclusive localization of tandem repetitive sequences in sulbtelomeric heterochromatin regions of Leymus racemosus (Poaceae, Triticeae)
AU - Kishii, Masahiro
AU - Nagaki, Kiyotaka
AU - Tsujimoto, Hisashi
AU - Sasakuma, Tetsuo
PY - 1999
Y1 - 1999
N2 - Two kinds of tandem repetitive sequences were isolated from Leymus racemosus (Lam.) Tzvelev. One of them was classified in the 350-bp family originally isolated from Secale. The other was a novel repetitive sequence family, named 'TaiI family', which consisted of a repeat unit of 570 bp. Fluorescence in-situ hybridization of the chromosomes of L. racemosus indicated that both families were located in subtelomeric heterochromatin and that the 350-bp family and TaiI family occupied different heterochromatin regions. In addition, even homologous chromosomes did not show the same patterns of TaiI and 350-bp families. The combination of these two families of repetitive sequences, together with Afa-family sequences and rDNAs, helps to identify the ten homologous chromosome pairs of L. racemosus. From these data, we proposed a karyotype of L. racemosus and compared it with other karyotypes already reported.
AB - Two kinds of tandem repetitive sequences were isolated from Leymus racemosus (Lam.) Tzvelev. One of them was classified in the 350-bp family originally isolated from Secale. The other was a novel repetitive sequence family, named 'TaiI family', which consisted of a repeat unit of 570 bp. Fluorescence in-situ hybridization of the chromosomes of L. racemosus indicated that both families were located in subtelomeric heterochromatin and that the 350-bp family and TaiI family occupied different heterochromatin regions. In addition, even homologous chromosomes did not show the same patterns of TaiI and 350-bp families. The combination of these two families of repetitive sequences, together with Afa-family sequences and rDNAs, helps to identify the ten homologous chromosome pairs of L. racemosus. From these data, we proposed a karyotype of L. racemosus and compared it with other karyotypes already reported.
KW - 350-bp family sequences
KW - Afa-family sequences
KW - Leymus
KW - Subtelomeric heterochromatin
KW - Triticeae
UR - http://www.scopus.com/inward/record.url?scp=0032751345&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032751345&partnerID=8YFLogxK
U2 - 10.1023/A:1009285311247
DO - 10.1023/A:1009285311247
M3 - Article
C2 - 10598567
AN - SCOPUS:0032751345
VL - 7
SP - 519
EP - 529
JO - Chromosome Research
JF - Chromosome Research
SN - 0967-3849
IS - 7
ER -