Excitatory and inhibitory postsynaptic currents of the superior salivatory nucleus innervating the salivary glands and tongue in the rat

Yoshihiro Mitoh, Makoto Funahashi, Motoi Kobashi, Ryuji Matsuo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The excitatory and inhibitory synaptic inputs to parasympathetic preganglionic neurons in the superior salivatory (SS) nucleus were investigated in brain slices of neonatal (4-8 days old) rat using the whole-cell patch-clamp technique. The SS neurons innervating the submandibular and sublingual salivary glands and innervating the lingual artery in the anterior region of the tongue were identified by retrograde transport of a fluorescent tracer. Whole-cell currents were evoked by electrical stimulation of tissue surrounding the cell. These evoked postsynaptic currents were completely abolished by antagonists for N-methyl-D-aspartate (NMDA) glutamate, non-NMDA glutamate, γ-aminobutyric acid type A (GABAA), and glycine receptors, suggesting that SS neurons receive glutamatergic excitatory, and GABAergic and glycinergic inhibitory synaptic inputs. In SS neurons for the salivary glands, the ratio of the NMDA component to the total excitatory postsynaptic current (EPSC) was larger than that of the non-NMDA component. This profile was reversed in the SS neurons for the tongue. In SS neurons for the salivary glands, the ratio of the GABAA component to the total IPSC was larger than the ratio of the glycine component to total inhibitory postsynaptic current (IPSC). The decay time constants of the GABAA component were slower than those for glycine. These characteristics of the excitatory and inhibitory inputs may be involved in determining the firing properties of the SS neurons innervating the salivary glands and the tongue.

Original languageEnglish
Pages (from-to)62-72
Number of pages11
JournalBrain Research
Volume999
Issue number1
DOIs
Publication statusPublished - Feb 27 2004

Fingerprint

Inhibitory Postsynaptic Potentials
Excitatory Postsynaptic Potentials
Salivary Glands
Tongue
Neurons
Aminobutyrates
D-Aspartic Acid
Glutamic Acid
N-Methylaspartate
Glycine
Sublingual Gland
Glycine Receptors
Synaptic Potentials
Patch-Clamp Techniques
Electric Stimulation
Arteries
Brain

Keywords

  • Patch-clamp
  • Superior salivatory nucleus
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Excitatory and inhibitory postsynaptic currents of the superior salivatory nucleus innervating the salivary glands and tongue in the rat. / Mitoh, Yoshihiro; Funahashi, Makoto; Kobashi, Motoi; Matsuo, Ryuji.

In: Brain Research, Vol. 999, No. 1, 27.02.2004, p. 62-72.

Research output: Contribution to journalArticle

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