Excitation relaxation dynamics and energy transfer in fucoxanthin- chlorophyll a/c-protein complexes, probed by time-resolved fluorescence

Seiji Akimoto, Ayaka Teshigahara, Makio Yokono, Mamoru Mimuro, Ryo Nagao, Tatsuya Tomo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In algae, light-harvesting complexes contain specific chlorophylls (Chls) and keto-carotenoids; Chl a, Chl c, and fucoxanthin (Fx) in diatoms and brown algae; Chl a, Chl c, and peridinin in photosynthetic dinoflagellates; and Chl a, Chl b, and siphonaxanthin in green algae. The Fx-Chl a/c-protein (FCP) complex from the diatom Chaetoceros gracilis contains Chl c1, Chl c 2, and the keto-carotenoid, Fx, as antenna pigments, in addition to Chl a. In the present study, we investigated energy transfer in the FCP complex associated with photosystem II (FCPII) of C. gracilis. For these investigations, we analyzed time-resolved fluorescence spectra, fluorescence rise and decay curves, and time-resolved fluorescence anisotropy data. Chl a exhibited different energy forms with fluorescence peaks ranging from 677 nm to 688 nm. Fx transferred excitation energy to lower-energy Chl a with a time constant of 300 fs. Chl c transferred excitation energy to Chl a with time constants of 500-600 fs (intra-complex transfer), 600-700 fs (intra-complex transfer), and 4-6 ps (inter-complex transfer). The latter process made a greater contribution to total Chl c-to-Chl a transfer in intact cells of C. gracilis than in the isolated FCPII complexes. The lower-energy Chl a received excitation energy from Fx and transferred the energy to higher-energy Chl a. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy.

Original languageEnglish
Pages (from-to)1514-1521
Number of pages8
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1837
Issue number9
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Chlorophyll Binding Proteins
Energy Transfer
Energy transfer
Fluorescence
Excitation energy
Algae
Diatoms
Photosystem II Protein Complex
Carotenoids
Proteins
Phaeophyta
Dinoflagellida
Chlorophyta
Fluorescence Polarization
Photosynthesis
Chlorophyll
Pigments
Sustainable development
Anisotropy
fucoxanthin

Keywords

  • Energy transfer
  • Fucoxanthin-chlorophyll a/c protein
  • Light harvesting
  • Photosynthesis
  • Time-resolved spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Excitation relaxation dynamics and energy transfer in fucoxanthin- chlorophyll a/c-protein complexes, probed by time-resolved fluorescence. / Akimoto, Seiji; Teshigahara, Ayaka; Yokono, Makio; Mimuro, Mamoru; Nagao, Ryo; Tomo, Tatsuya.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1837, No. 9, 2014, p. 1514-1521.

Research output: Contribution to journalArticle

Akimoto, Seiji ; Teshigahara, Ayaka ; Yokono, Makio ; Mimuro, Mamoru ; Nagao, Ryo ; Tomo, Tatsuya. / Excitation relaxation dynamics and energy transfer in fucoxanthin- chlorophyll a/c-protein complexes, probed by time-resolved fluorescence. In: Biochimica et Biophysica Acta - Bioenergetics. 2014 ; Vol. 1837, No. 9. pp. 1514-1521.
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AU - Akimoto, Seiji

AU - Teshigahara, Ayaka

AU - Yokono, Makio

AU - Mimuro, Mamoru

AU - Nagao, Ryo

AU - Tomo, Tatsuya

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AB - In algae, light-harvesting complexes contain specific chlorophylls (Chls) and keto-carotenoids; Chl a, Chl c, and fucoxanthin (Fx) in diatoms and brown algae; Chl a, Chl c, and peridinin in photosynthetic dinoflagellates; and Chl a, Chl b, and siphonaxanthin in green algae. The Fx-Chl a/c-protein (FCP) complex from the diatom Chaetoceros gracilis contains Chl c1, Chl c 2, and the keto-carotenoid, Fx, as antenna pigments, in addition to Chl a. In the present study, we investigated energy transfer in the FCP complex associated with photosystem II (FCPII) of C. gracilis. For these investigations, we analyzed time-resolved fluorescence spectra, fluorescence rise and decay curves, and time-resolved fluorescence anisotropy data. Chl a exhibited different energy forms with fluorescence peaks ranging from 677 nm to 688 nm. Fx transferred excitation energy to lower-energy Chl a with a time constant of 300 fs. Chl c transferred excitation energy to Chl a with time constants of 500-600 fs (intra-complex transfer), 600-700 fs (intra-complex transfer), and 4-6 ps (inter-complex transfer). The latter process made a greater contribution to total Chl c-to-Chl a transfer in intact cells of C. gracilis than in the isolated FCPII complexes. The lower-energy Chl a received excitation energy from Fx and transferred the energy to higher-energy Chl a. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy.

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