Excellent islet yields after 18-h porcine pancreas preservation by ductal injection, pancreas preservation with MK solution, bottle purification, and islet purification using iodixanol with UW solution and iodixanol with MK solution

Kazuho Kuwae, Chika Miyagi-Shiohira, Eri Hamada, Yoshihito Tamaki, Kai Nishime, Mayuko Sakai, Tasuku Yonaha, Erika Makishi, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Successful islet isolation is the key to successful islet transplantation. Our group recently modified the islet isolation protocol to include pancreatic ductal injection of the preservation solution, pancreas storage in modified extracellular-type trehalose-containing Kyoto (MK) solution, and use of an iodixanol-based purification solution and bottle purification. In this study, we applied these methods to porcine islet isolation after 18-h pancreas preservation and compared two solutions with different compositions in bottle purification. Islet yield before purification was 651,661 ± 157,719 islet equivalents (IE) and 5576 ± 1538 IE/g pancreas weight. An IU solution was made by adding iodixanol to University of Wisconsin solution and an IK solution was made by adding iodixanol to MK solution. The efficacy of the two solutions for islet isolation was compared. There were no significant differences between the two purification methods with regard to islet yield, survival rate, purity, score, or stimulation index. These results indicate that our isolation protocol produces efficient islet yields from prolonged cold-stored pancreas and that IU and IK solutions are equally useful for islet purification.

Original languageEnglish
Article number1561
JournalJournal of Clinical Medicine
Volume8
Issue number10
DOIs
Publication statusPublished - Oct 2019

Keywords

  • Extracellular-type trehalose-containing Kyoto (ETK) solution
  • Iodixanol
  • Islet isolation
  • Islet purification
  • Islet transplantation
  • Modified ETK (MK) solution
  • University of Wisconsin solution

ASJC Scopus subject areas

  • Medicine(all)

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