Examination of transpositional activity of nDart1at different stages of rice development

Mika Hayashi-Tsugane, Masahiko Maekawa, Hirokazu Kobayashi, Shigeru Iida, Kazuo Tsugane

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

As a useful tool to elucidate gene functions, a rice transposon tagging line has been developed using an active endogenous DNA transposon, nDart1. It was highly desirable to evaluate the transposition timing and frequency of the nDart1elements during rice development to facilitate the generation of an efficient mutant isolation system. Comparison of the detected new insertions at different stages of rice development by transposon display analysis demonstrated that the last heading tiller carry a higher number of nDart1 elements than the main culm. Moreover, it was revealed that the last heading tiller could produce progeny that carried more new insertions of nDart1 elements, mainly as a result of the accumulation of somatic insertions in the parental plant. This report demonstrates that late tillers increase the probability of producing independent mutant lines.

Original languageEnglish
Pages (from-to)215-219
Number of pages5
JournalGenes and Genetic Systems
Volume86
Issue number3
DOIs
Publication statusPublished - Oct 4 2011

Keywords

  • DNA transposon
  • Gene tagging
  • Tiller
  • Timing of transposition
  • Transposon display

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Examination of transpositional activity of nDart1at different stages of rice development'. Together they form a unique fingerprint.

  • Cite this

    Hayashi-Tsugane, M., Maekawa, M., Kobayashi, H., Iida, S., & Tsugane, K. (2011). Examination of transpositional activity of nDart1at different stages of rice development. Genes and Genetic Systems, 86(3), 215-219. https://doi.org/10.1266/ggs.86.215