Examination of drug resistance activity of human TAP-like (ABCB9) expressed in yeast

Ayako Ohashi-Kobayashi, Kazuaki Ohashi, Wei Bin Du, Hiroshi Omote, Robert Nakamoto, Marwan Al-Shawi, Masatomo Maeda

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A half-type ABC transporter, human TAP-like (hTAPL) tagged with histidine cluster, was expressed in budding yeast protease-deficient strain BJ5457, and the effect of expression for resistance to peptide compounds including antibiotics and proteinase inhibitor was examined. Among these compounds, the yeast expressing hTAPL exhibits high sensitivity to valinomycin, a monovalent cation ionophore. A mutation in Walker A motif, which lost ATP-binding activity of hTAPL, eliminated the enhanced sensitivity to valinomycin. These findings suggest that the transport activity of hTAPL is important for conferring high valinomycin-sensitive phenotype to yeast.

Original languageEnglish
Pages (from-to)597-601
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume343
Issue number2
DOIs
Publication statusPublished - May 5 2006
Externally publishedYes

Keywords

  • ATP-binding cassette transporter
  • MDR
  • TAPL
  • Valinomycin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Examination of drug resistance activity of human TAP-like (ABCB9) expressed in yeast'. Together they form a unique fingerprint.

Cite this