TY - JOUR
T1 - Examination of drug resistance activity of human TAP-like (ABCB9) expressed in yeast
AU - Ohashi-Kobayashi, Ayako
AU - Ohashi, Kazuaki
AU - Du, Wei Bin
AU - Omote, Hiroshi
AU - Nakamoto, Robert
AU - Al-Shawi, Marwan
AU - Maeda, Masatomo
N1 - Funding Information:
We thank Y. Kezuka for technical assistance. We also thank Y. Nakajima and H. Tsukamoto for helping early stage of this experiment. Original MDR construct is a kind gift of M. Gottesmann (NIH). This research was supported in part by grants from MEXT [Grants-in-Aid for Scientific Research C (13672284)], JSPS [Cooperative Research under the Japan-U.S. Cooperative Science Program, and Grants-in-Aid for Scientific Research on Priority Areas A (13033027) and Priority Areas B (13142206)], and the Mochida Research Foundation (to A.K.), IYAKUSHIGEN (to A.K.), and Hoansya (to M.M.).
PY - 2006/5/5
Y1 - 2006/5/5
N2 - A half-type ABC transporter, human TAP-like (hTAPL) tagged with histidine cluster, was expressed in budding yeast protease-deficient strain BJ5457, and the effect of expression for resistance to peptide compounds including antibiotics and proteinase inhibitor was examined. Among these compounds, the yeast expressing hTAPL exhibits high sensitivity to valinomycin, a monovalent cation ionophore. A mutation in Walker A motif, which lost ATP-binding activity of hTAPL, eliminated the enhanced sensitivity to valinomycin. These findings suggest that the transport activity of hTAPL is important for conferring high valinomycin-sensitive phenotype to yeast.
AB - A half-type ABC transporter, human TAP-like (hTAPL) tagged with histidine cluster, was expressed in budding yeast protease-deficient strain BJ5457, and the effect of expression for resistance to peptide compounds including antibiotics and proteinase inhibitor was examined. Among these compounds, the yeast expressing hTAPL exhibits high sensitivity to valinomycin, a monovalent cation ionophore. A mutation in Walker A motif, which lost ATP-binding activity of hTAPL, eliminated the enhanced sensitivity to valinomycin. These findings suggest that the transport activity of hTAPL is important for conferring high valinomycin-sensitive phenotype to yeast.
KW - ATP-binding cassette transporter
KW - MDR
KW - TAPL
KW - Valinomycin
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U2 - 10.1016/j.bbrc.2006.03.002
DO - 10.1016/j.bbrc.2006.03.002
M3 - Article
C2 - 16554024
AN - SCOPUS:33645116842
SN - 0006-291X
VL - 343
SP - 597
EP - 601
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -