Ex vivo proteomics of Campylobacter jejuni 81-176 reveal that FabG affects fatty acid composition to alter bacterial growth fitness in the chicken gut

Hiroshi Asakura, Keiko Kawamoto, Satoshi Murakami, Masato Tachibana, Hisao Kurazono, Sou Ichi Makino, Shigeki Yamamoto, Shizunobu Igimi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Campylobacter jejuni is one of the leading causes of foodborne gastrointestinal illness worldwide. Here we performed ex vivo proteomic analysis of C. jejuni 81-176 in chicken, a main reservoir for human infection. At 0, 1 and 4 weeks post-infection (p.i.) with the GFP-expressing 81-176 strain, inocula were recovered from chicken ceca by cell sorting using flow cytometry. iTRAQ-coupled 2D-LC-MS/MS analyses that detected 55 C. jejuni proteins, among which either 3 (FabG, HydB, CJJ81176_0876) or 7 (MscS, CetB, FlhF, PurH, PglJ, LpxC, Icd) proteins exhibited >1.4-fold-increased expression at 1 or 4 week(s) p.i. compared with those at 0 weeks p.i., respectively. Deletion of the fabG gene clearly decreased the proportion of bacterial unsaturated fatty acids (UFAs) and chicken colonization. The UFA proportion of the parental strain was not altered when grown at 42 °C. These findings suggest that FabG might play a pivotal role in UFA production, linked to bacterial adaptation in the poultry host. To our knowledge, this is the first example of ex vivo C. jejuni proteomics, in which fatty acid metabolism might affect bacterial adaptation to the chicken host.

Original languageEnglish
Pages (from-to)63-71
Number of pages9
JournalResearch in Microbiology
Volume167
Issue number2
DOIs
Publication statusPublished - Feb 1 2016
Externally publishedYes

Keywords

  • Campylobacter jejuni
  • Chicken colonization
  • Fatty acid biosynthesis
  • LC-MS

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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