Ex vivo generation of a functional and regenerative wound epithelium from axolotl (Ambystoma mexicanum) skin

Donald R. Ferris, Akira Sato, Berhan Mandefro, Gillian M. Cummings, David M. Gardiner, Elizabeth L. Rugg

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Urodele amphibians (salamanders) are unique among adult vertebrates in their ability to regenerate structurally complete and fully functional limbs. Regeneration is a stepwise process that requires interactions between keratinocytes, nerves and fibroblasts. The formation of a wound epithelium covering the amputation site is an early and necessary event in the process but the molecular mechanisms that underlie the role of the wound epithelium in regeneration remain unclear. We have developed an ex vivo model that recapitulates many features of in vivo wound healing. The model comprises a circular explant of axolotl (Ambystoma mexicanum) limb skin with a central circular, full thickness wound. Re-epithelialization of the wound area is rapid (typically

Original languageEnglish
Pages (from-to)715-724
Number of pages10
JournalDevelopment Growth and Differentiation
Volume52
Issue number8
DOIs
Publication statusPublished - Oct 2010
Externally publishedYes

Fingerprint

Ambystoma mexicanum
Epithelium
Skin
Wounds and Injuries
Regeneration
Extremities
Re-Epithelialization
Urodela
Amphibians
Keratinocytes
Amputation
Wound Healing
Vertebrates
Fibroblasts

Keywords

  • axolotl
  • blastema
  • limb regeneration
  • matrix metalloproteinase
  • wound epithelium

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Ex vivo generation of a functional and regenerative wound epithelium from axolotl (Ambystoma mexicanum) skin. / Ferris, Donald R.; Sato, Akira; Mandefro, Berhan; Cummings, Gillian M.; Gardiner, David M.; Rugg, Elizabeth L.

In: Development Growth and Differentiation, Vol. 52, No. 8, 10.2010, p. 715-724.

Research output: Contribution to journalArticle

Ferris, Donald R. ; Sato, Akira ; Mandefro, Berhan ; Cummings, Gillian M. ; Gardiner, David M. ; Rugg, Elizabeth L. / Ex vivo generation of a functional and regenerative wound epithelium from axolotl (Ambystoma mexicanum) skin. In: Development Growth and Differentiation. 2010 ; Vol. 52, No. 8. pp. 715-724.
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