Abstract
In order to understand the molecular evolution of catalase genes in higher plants, we compared the exon-intron structures of 12 genomic sequences from six plant species. It was assumed that the putative single primordial catalase gene had seven introns, because only those catalase genes having this structure are found in the monocotyledonae and dicotyledonae classes. After the evolutionary divergence of monocots from dicots, consecutive duplication of the primordial gene followed by the differential loss of introns occurred in each class to form three (or possibly four in dicots) diverse isozyme genes. In monocots, three ancestral isozyme genes were formed before the divergence of ancestral rice and maize. One of the rice genes, CatA, has an entirely new short intron which was not found in any other plant catalase gene examined. We have investigated the existence of the intron in the CatA homolog in other rice species by polymerase chain reaction (PCR) analysis. One major PCR product was found with the genomic DNAs from O. sativa (indica and japonica types), O. rufipogon and O. glaberrima. DNAs from several accessions of O. longistaminata showed variation in both the number and size of the DNA fragments amplified. PCR analyses and sequencing of the PCR products revealed that there are several CatA homologs having different sequences in some accessions of O. longistaminata. We have extended our study to other species in the Poaceae. The results suggest that the gain of the intron, most likely by insertion of a retroposon, took place in the ancestral genome of rice after its evolutionary divergence from other ancestral cereals such as barley, wheat and oat.
| Original language | English |
|---|---|
| Pages (from-to) | 9-19 |
| Number of pages | 11 |
| Journal | Theoretical And Applied Genetics |
| Volume | 97 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Jul 1998 |
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Keywords
- Catalase
- Evolution
- Gene structure
- Rice
- Transposon
ASJC Scopus subject areas
- Agronomy and Crop Science
- Horticulture
- Plant Science
- Genetics
- Genetics(clinical)
Cite this
Evolutionary relationship of plant catalase genes inferred from exon-intron structures : Isozyme divergence after the separation of monocots and dicots. / Iwamoto, M.; Maekawa, Masahiko; Saito, A.; Higo, H.; Higo, K.
In: Theoretical And Applied Genetics, Vol. 97, No. 1-2, 07.1998, p. 9-19.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evolutionary relationship of plant catalase genes inferred from exon-intron structures
T2 - Isozyme divergence after the separation of monocots and dicots
AU - Iwamoto, M.
AU - Maekawa, Masahiko
AU - Saito, A.
AU - Higo, H.
AU - Higo, K.
PY - 1998/7
Y1 - 1998/7
N2 - In order to understand the molecular evolution of catalase genes in higher plants, we compared the exon-intron structures of 12 genomic sequences from six plant species. It was assumed that the putative single primordial catalase gene had seven introns, because only those catalase genes having this structure are found in the monocotyledonae and dicotyledonae classes. After the evolutionary divergence of monocots from dicots, consecutive duplication of the primordial gene followed by the differential loss of introns occurred in each class to form three (or possibly four in dicots) diverse isozyme genes. In monocots, three ancestral isozyme genes were formed before the divergence of ancestral rice and maize. One of the rice genes, CatA, has an entirely new short intron which was not found in any other plant catalase gene examined. We have investigated the existence of the intron in the CatA homolog in other rice species by polymerase chain reaction (PCR) analysis. One major PCR product was found with the genomic DNAs from O. sativa (indica and japonica types), O. rufipogon and O. glaberrima. DNAs from several accessions of O. longistaminata showed variation in both the number and size of the DNA fragments amplified. PCR analyses and sequencing of the PCR products revealed that there are several CatA homologs having different sequences in some accessions of O. longistaminata. We have extended our study to other species in the Poaceae. The results suggest that the gain of the intron, most likely by insertion of a retroposon, took place in the ancestral genome of rice after its evolutionary divergence from other ancestral cereals such as barley, wheat and oat.
AB - In order to understand the molecular evolution of catalase genes in higher plants, we compared the exon-intron structures of 12 genomic sequences from six plant species. It was assumed that the putative single primordial catalase gene had seven introns, because only those catalase genes having this structure are found in the monocotyledonae and dicotyledonae classes. After the evolutionary divergence of monocots from dicots, consecutive duplication of the primordial gene followed by the differential loss of introns occurred in each class to form three (or possibly four in dicots) diverse isozyme genes. In monocots, three ancestral isozyme genes were formed before the divergence of ancestral rice and maize. One of the rice genes, CatA, has an entirely new short intron which was not found in any other plant catalase gene examined. We have investigated the existence of the intron in the CatA homolog in other rice species by polymerase chain reaction (PCR) analysis. One major PCR product was found with the genomic DNAs from O. sativa (indica and japonica types), O. rufipogon and O. glaberrima. DNAs from several accessions of O. longistaminata showed variation in both the number and size of the DNA fragments amplified. PCR analyses and sequencing of the PCR products revealed that there are several CatA homologs having different sequences in some accessions of O. longistaminata. We have extended our study to other species in the Poaceae. The results suggest that the gain of the intron, most likely by insertion of a retroposon, took place in the ancestral genome of rice after its evolutionary divergence from other ancestral cereals such as barley, wheat and oat.
KW - Catalase
KW - Evolution
KW - Gene structure
KW - Rice
KW - Transposon
UR - http://www.scopus.com/inward/record.url?scp=0031844194&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031844194&partnerID=8YFLogxK
U2 - 10.1007/s001220050861
DO - 10.1007/s001220050861
M3 - Article
AN - SCOPUS:0031844194
VL - 97
SP - 9
EP - 19
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
SN - 0040-5752
IS - 1-2
ER -