Evolutionary changes of the flhDC flagellar master operon in Shigella strains

Akira Tominaga, Ruiting Lan, Peter R. Reeves

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Shigella strains are nonmotile. The master operon of flagellar synthesis, flhDC, was analyzed for genetic damage in 46 Shigella strains representing all known serotypes. In 11 strains (B1, B3, B6, B8, B10, B18, D5, F1B, D10, F3A, and F3C) the flhDC operon was completely deleted. PCR and sequence analysis of the flhDC region of the remaining 35 strains revealed many insertions or deletions associated with insertion sequences, and the majority of the strains were found to be defective in their flhDC genes. As these genes also play a role in regulation of nonflagellar genes, the loss may have other consequences or be driven by selection pressures other than those against flagellar motility. It has been suggested that Shigella strains fall mostly into three clusters within Escherichla coli, with five outlier strains, four of which are also within E. coli (G. M. Pupo, R. Lan, and P. R. Reeves, Proc. Natl. Acad. Sci. USA 97:10567-10572, 2000). The distribution of genetic changes in the flhDC region correlated very well with the three clusters and outlier strains found using housekeeping gene DNA sequences, enabling us to follow the sequence of mutational change in the flhDC locus. Two cluster 2 strains were found to have unique flhDC sequences, which are most probably due to recombination during the exchange of the adjacent O-antigen gene clusters.

Original languageEnglish
Pages (from-to)4295-4302
Number of pages8
JournalJournal of Bacteriology
Volume187
Issue number12
DOIs
Publication statusPublished - Jun 2005

Fingerprint

Shigella
Operon
Genes
O Antigens
Insertional Mutagenesis
Essential Genes
Multigene Family
Genetic Recombination
Sequence Analysis
Escherichia coli
Pressure
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Evolutionary changes of the flhDC flagellar master operon in Shigella strains. / Tominaga, Akira; Lan, Ruiting; Reeves, Peter R.

In: Journal of Bacteriology, Vol. 187, No. 12, 06.2005, p. 4295-4302.

Research output: Contribution to journalArticle

Tominaga, Akira ; Lan, Ruiting ; Reeves, Peter R. / Evolutionary changes of the flhDC flagellar master operon in Shigella strains. In: Journal of Bacteriology. 2005 ; Vol. 187, No. 12. pp. 4295-4302.
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