Evidence that temporally alternative expression of the Vibrio vulnificus elastase prevents proteolytic inactivation of hemolysin

Jee Eun Rhee, Jeong Hyun Lee, Hye Sook Jeong, Uryung Park, Dong Ha Lee, Gun Jo Woo, Shin-ichi Miyoshi, Sang Ho Choi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Numerous secreted and cell-associated virulence factors have been proposed to account for the fulminating and destructive nature of Vibrio vulnificus infections. Among the putative virulence factors are an elastase, elastolytic protease, and a cytolytic hemolysin. Effects of the elastase on the hemolysin were assessed by evaluating changes of hemolytic activities either in the presence or absence of the protease. Although hemolytic activity in the culture supernatant was lowered by the purified elastase added in vitro, the cellular level of hemolytic activity was unaffected by the mutation of ννpE encoding the elastase. Growth kinetic studies revealed that hemolysin reached its maximum level in the exponential phase of growth, and the elastase appeared at the onset of the stationary phase. These results have provided insight into the regulation of virulence factors: temporally coordinate regulation of virulence factors is essential for the overall success of the pathogen during pathogenesis.

Original languageEnglish
Pages (from-to)1021-1026
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume13
Issue number6
Publication statusPublished - Dec 2003

Fingerprint

Vibrio vulnificus
Hemolysin Proteins
Pancreatic Elastase
Virulence Factors
Growth kinetics
Pathogens
Peptide Hydrolases
Growth
Mutation

Keywords

  • Elastase
  • Hemolysin
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Rhee, J. E., Lee, J. H., Jeong, H. S., Park, U., Lee, D. H., Woo, G. J., ... Choi, S. H. (2003). Evidence that temporally alternative expression of the Vibrio vulnificus elastase prevents proteolytic inactivation of hemolysin. Journal of Microbiology and Biotechnology, 13(6), 1021-1026.

Evidence that temporally alternative expression of the Vibrio vulnificus elastase prevents proteolytic inactivation of hemolysin. / Rhee, Jee Eun; Lee, Jeong Hyun; Jeong, Hye Sook; Park, Uryung; Lee, Dong Ha; Woo, Gun Jo; Miyoshi, Shin-ichi; Choi, Sang Ho.

In: Journal of Microbiology and Biotechnology, Vol. 13, No. 6, 12.2003, p. 1021-1026.

Research output: Contribution to journalArticle

Rhee, Jee Eun ; Lee, Jeong Hyun ; Jeong, Hye Sook ; Park, Uryung ; Lee, Dong Ha ; Woo, Gun Jo ; Miyoshi, Shin-ichi ; Choi, Sang Ho. / Evidence that temporally alternative expression of the Vibrio vulnificus elastase prevents proteolytic inactivation of hemolysin. In: Journal of Microbiology and Biotechnology. 2003 ; Vol. 13, No. 6. pp. 1021-1026.
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