Evidence that RNA silencing-mediated resistance to Beet necrotic yellow vein virus is less effective in roots than in leaves

Andika Ida Bagus, Hideki Kondo, Tetsuo Tamada

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

In plants, RNA silencing is part of a defense mechanism against virus infection but there is little information as to whether RNA silencing-mediated resistance functions similarly in roots and leaves. We have obtained transgenic Nicotiana benthamiana plants encoding the coat protein readthrough domain open reading frame (54 kDa) of Beet necrotic yellow vein virus (BNYVV), which either showed a highly resistant or a recovery phenotype following foliar rub-inoculation with BNYVV. These phenotypes were associated with an RNA silencing mechanism. Roots of the resistant plants that were immune to foliar rub-inoculation with BNYVV could be infected by viruliferous zoospores of the vector fungus Polymyxa betae, although virus multiplication was greatly limited. In addition, virus titer was reduced in symptomless leaves of the plants showing the recovery phenotype, but it was high in roots of the same plants. Compared with leaves of silenced plants, higher levels of transgene mRNAs and lower levels of transgene-derived small interfering RNAs (siRNAs) accumulated in roots. Similarly, in nontransgenic plants inoculated with BNYVV, accumulation level of viral RNA-derived siRNAs in roots was lower than in leaves. These results indicate that the RNA silencing-mediated resistance to BNYVV is less effective in roots than in leaves.

Original languageEnglish
Pages (from-to)194-204
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume18
Issue number3
DOIs
Publication statusPublished - Mar 2005

Fingerprint

Beet necrotic yellow vein virus
Beta vulgaris
RNA Interference
RNA interference
Viruses
Veins
RNA
leaves
Plant Roots
Plant Leaves
small interfering RNA
Transgenes
Phenotype
phenotype
Small Interfering RNA
transgenes
Polymyxa betae
Plasmodiophorida
Plant RNA
viruses

Keywords

  • Benyvirus

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Evidence that RNA silencing-mediated resistance to Beet necrotic yellow vein virus is less effective in roots than in leaves. / Ida Bagus, Andika; Kondo, Hideki; Tamada, Tetsuo.

In: Molecular Plant-Microbe Interactions, Vol. 18, No. 3, 03.2005, p. 194-204.

Research output: Contribution to journalArticle

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