Evidence of oncotic cell death and DNA fragmentation in human hypertropic chondrocytes in chondro-osteophyte

T. Doi, Keiichiro Nishida, M. Matsuo, A. Yoshida, T. Murakami, H. Inoue

Research output: Contribution to journalArticle

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Abstract

Objective: To investigate the population and morphology of in situ terminal deoxynucleotidyle transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) stain positive non-apoptotic chondrocytes in hypertrophic zone of human chondro-osteophytes. Materials and Methods: Chondro-osteophytes from osteoarthritic patients were obtained at joint replacement surgery. Apoptosis was verified by light microscopic examination of Safranin O stained sections and TUNEL stain. TUNEL staining was also performed on hydrophilic resin embedded semi-thin and ultra-thin sections combined with the treatment with streptavidin-gold conjugates, observed by light microscopy with silver enhancement technique (TUNEL-LM with SE) and transmission electron microscopy (TUNEL-TEM) respectively for the simultaneous evaluation of cellular structure and DNA fragmentation. Results: In paraffin embedded sections (N=18), 31.5±6.1% of cells in the hypertrophic zone were TUNEL positive, but only 3.8±1.2% cells in this zone showed apoptotic appearances with cell shrinkage and nuclear condensation. Both in TUNEL-TEM and TUNEL-LM with SE, gold particles, which indicate DNA fragmentation, were observed within the nucleus of morphologically apoptotic chondrocytes, as well as of disintegrated, swollen chondrocytes. Conclusions: In human chondro-osteophytes, hypertrophic chondrocytes might die by oncotic cell death with DNA fragmentation, as well as apoptosis.

Original languageEnglish
Pages (from-to)270-276
Number of pages7
JournalOsteoarthritis and Cartilage
Volume10
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Osteophyte
DNA Fragmentation
Cell death
Chondrocytes
Labeling
DNA
Cell Death
Gold
Replacement Arthroplasties
Coloring Agents
Apoptosis
Light
Streptavidin
Cellular Structures
Transferases
Transmission Electron Microscopy
Silver
Paraffin
Microscopy
Transmission electron microscopy

Keywords

  • Apoptosis
  • Chondro-osteophyte
  • Chondrocyte
  • Enchondral ossification
  • Oncosis

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Evidence of oncotic cell death and DNA fragmentation in human hypertropic chondrocytes in chondro-osteophyte. / Doi, T.; Nishida, Keiichiro; Matsuo, M.; Yoshida, A.; Murakami, T.; Inoue, H.

In: Osteoarthritis and Cartilage, Vol. 10, No. 4, 2002, p. 270-276.

Research output: Contribution to journalArticle

Doi, T. ; Nishida, Keiichiro ; Matsuo, M. ; Yoshida, A. ; Murakami, T. ; Inoue, H. / Evidence of oncotic cell death and DNA fragmentation in human hypertropic chondrocytes in chondro-osteophyte. In: Osteoarthritis and Cartilage. 2002 ; Vol. 10, No. 4. pp. 270-276.
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abstract = "Objective: To investigate the population and morphology of in situ terminal deoxynucleotidyle transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) stain positive non-apoptotic chondrocytes in hypertrophic zone of human chondro-osteophytes. Materials and Methods: Chondro-osteophytes from osteoarthritic patients were obtained at joint replacement surgery. Apoptosis was verified by light microscopic examination of Safranin O stained sections and TUNEL stain. TUNEL staining was also performed on hydrophilic resin embedded semi-thin and ultra-thin sections combined with the treatment with streptavidin-gold conjugates, observed by light microscopy with silver enhancement technique (TUNEL-LM with SE) and transmission electron microscopy (TUNEL-TEM) respectively for the simultaneous evaluation of cellular structure and DNA fragmentation. Results: In paraffin embedded sections (N=18), 31.5±6.1{\%} of cells in the hypertrophic zone were TUNEL positive, but only 3.8±1.2{\%} cells in this zone showed apoptotic appearances with cell shrinkage and nuclear condensation. Both in TUNEL-TEM and TUNEL-LM with SE, gold particles, which indicate DNA fragmentation, were observed within the nucleus of morphologically apoptotic chondrocytes, as well as of disintegrated, swollen chondrocytes. Conclusions: In human chondro-osteophytes, hypertrophic chondrocytes might die by oncotic cell death with DNA fragmentation, as well as apoptosis.",
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AU - Matsuo, M.

AU - Yoshida, A.

AU - Murakami, T.

AU - Inoue, H.

PY - 2002

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N2 - Objective: To investigate the population and morphology of in situ terminal deoxynucleotidyle transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) stain positive non-apoptotic chondrocytes in hypertrophic zone of human chondro-osteophytes. Materials and Methods: Chondro-osteophytes from osteoarthritic patients were obtained at joint replacement surgery. Apoptosis was verified by light microscopic examination of Safranin O stained sections and TUNEL stain. TUNEL staining was also performed on hydrophilic resin embedded semi-thin and ultra-thin sections combined with the treatment with streptavidin-gold conjugates, observed by light microscopy with silver enhancement technique (TUNEL-LM with SE) and transmission electron microscopy (TUNEL-TEM) respectively for the simultaneous evaluation of cellular structure and DNA fragmentation. Results: In paraffin embedded sections (N=18), 31.5±6.1% of cells in the hypertrophic zone were TUNEL positive, but only 3.8±1.2% cells in this zone showed apoptotic appearances with cell shrinkage and nuclear condensation. Both in TUNEL-TEM and TUNEL-LM with SE, gold particles, which indicate DNA fragmentation, were observed within the nucleus of morphologically apoptotic chondrocytes, as well as of disintegrated, swollen chondrocytes. Conclusions: In human chondro-osteophytes, hypertrophic chondrocytes might die by oncotic cell death with DNA fragmentation, as well as apoptosis.

AB - Objective: To investigate the population and morphology of in situ terminal deoxynucleotidyle transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) stain positive non-apoptotic chondrocytes in hypertrophic zone of human chondro-osteophytes. Materials and Methods: Chondro-osteophytes from osteoarthritic patients were obtained at joint replacement surgery. Apoptosis was verified by light microscopic examination of Safranin O stained sections and TUNEL stain. TUNEL staining was also performed on hydrophilic resin embedded semi-thin and ultra-thin sections combined with the treatment with streptavidin-gold conjugates, observed by light microscopy with silver enhancement technique (TUNEL-LM with SE) and transmission electron microscopy (TUNEL-TEM) respectively for the simultaneous evaluation of cellular structure and DNA fragmentation. Results: In paraffin embedded sections (N=18), 31.5±6.1% of cells in the hypertrophic zone were TUNEL positive, but only 3.8±1.2% cells in this zone showed apoptotic appearances with cell shrinkage and nuclear condensation. Both in TUNEL-TEM and TUNEL-LM with SE, gold particles, which indicate DNA fragmentation, were observed within the nucleus of morphologically apoptotic chondrocytes, as well as of disintegrated, swollen chondrocytes. Conclusions: In human chondro-osteophytes, hypertrophic chondrocytes might die by oncotic cell death with DNA fragmentation, as well as apoptosis.

KW - Apoptosis

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KW - Enchondral ossification

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