TY - JOUR
T1 - Evidence of oncotic cell death and DNA fragmentation in human hypertropic chondrocytes in chondro-osteophyte
AU - Doi, T.
AU - Nishida, K.
AU - Matsuo, M.
AU - Yoshida, A.
AU - Murakami, T.
AU - Inoue, H.
N1 - Funding Information:
The authors wish to thank Mr N. Kishimoto in the Central Research Laboratory of Okayama University Medical School for technical assistance. We also thank Mrs Cherie McCown for editing drafts of the manuscript. This work was supported in part by a grant-in aid to KN from the Ministry of Education, Science, Sports and Culture, Japan (11770803).
PY - 2002
Y1 - 2002
N2 - Objective: To investigate the population and morphology of in situ terminal deoxynucleotidyle transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) stain positive non-apoptotic chondrocytes in hypertrophic zone of human chondro-osteophytes. Materials and Methods: Chondro-osteophytes from osteoarthritic patients were obtained at joint replacement surgery. Apoptosis was verified by light microscopic examination of Safranin O stained sections and TUNEL stain. TUNEL staining was also performed on hydrophilic resin embedded semi-thin and ultra-thin sections combined with the treatment with streptavidin-gold conjugates, observed by light microscopy with silver enhancement technique (TUNEL-LM with SE) and transmission electron microscopy (TUNEL-TEM) respectively for the simultaneous evaluation of cellular structure and DNA fragmentation. Results: In paraffin embedded sections (N=18), 31.5±6.1% of cells in the hypertrophic zone were TUNEL positive, but only 3.8±1.2% cells in this zone showed apoptotic appearances with cell shrinkage and nuclear condensation. Both in TUNEL-TEM and TUNEL-LM with SE, gold particles, which indicate DNA fragmentation, were observed within the nucleus of morphologically apoptotic chondrocytes, as well as of disintegrated, swollen chondrocytes. Conclusions: In human chondro-osteophytes, hypertrophic chondrocytes might die by oncotic cell death with DNA fragmentation, as well as apoptosis.
AB - Objective: To investigate the population and morphology of in situ terminal deoxynucleotidyle transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) stain positive non-apoptotic chondrocytes in hypertrophic zone of human chondro-osteophytes. Materials and Methods: Chondro-osteophytes from osteoarthritic patients were obtained at joint replacement surgery. Apoptosis was verified by light microscopic examination of Safranin O stained sections and TUNEL stain. TUNEL staining was also performed on hydrophilic resin embedded semi-thin and ultra-thin sections combined with the treatment with streptavidin-gold conjugates, observed by light microscopy with silver enhancement technique (TUNEL-LM with SE) and transmission electron microscopy (TUNEL-TEM) respectively for the simultaneous evaluation of cellular structure and DNA fragmentation. Results: In paraffin embedded sections (N=18), 31.5±6.1% of cells in the hypertrophic zone were TUNEL positive, but only 3.8±1.2% cells in this zone showed apoptotic appearances with cell shrinkage and nuclear condensation. Both in TUNEL-TEM and TUNEL-LM with SE, gold particles, which indicate DNA fragmentation, were observed within the nucleus of morphologically apoptotic chondrocytes, as well as of disintegrated, swollen chondrocytes. Conclusions: In human chondro-osteophytes, hypertrophic chondrocytes might die by oncotic cell death with DNA fragmentation, as well as apoptosis.
KW - Apoptosis
KW - Chondro-osteophyte
KW - Chondrocyte
KW - Enchondral ossification
KW - Oncosis
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U2 - 10.1053/joca.2001.0503
DO - 10.1053/joca.2001.0503
M3 - Article
C2 - 11950249
AN - SCOPUS:0036256263
SN - 1063-4584
VL - 10
SP - 270
EP - 276
JO - Osteoarthritis and Cartilage
JF - Osteoarthritis and Cartilage
IS - 4
ER -