Evidence in vivo for autogenous control of the cyclic AMP receptor protein gene (crp) in Escherichia coli by divergent RNA.

K. Okamoto, S. Hara, R. Bhasin, M. Freundlich

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Control of crp expression in vivo was studied by using the cloned crp gene. The synthesis of the product of this gene, cyclic AMP (cAMP) receptor protein (CRP), was strongly reduced by exogenous cAMP. This regulation was completely abolished by the inactivation of a divergent promoter located within the crp promoter region. These data are consistent with our in vitro studies (Okamoto and Freundlich, Proc.Natl. Acad. Sci. USA 83:5000-5004, 1986), which showed that crp autoregulation is due to the inhibition of crp transcription by divergent (antisense) RNA produced by cAMP-CRP activation of the divergent promoter.

Original languageEnglish
Pages (from-to)5076-5079
Number of pages4
JournalJournal of bacteriology
Volume170
Issue number11
DOIs
Publication statusPublished - Nov 1988
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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