TY - JOUR
T1 - Evidence in vivo for autogenous control of the cyclic AMP receptor protein gene (crp) in Escherichia coli by divergent RNA.
AU - Okamoto, K.
AU - Hara, S.
AU - Bhasin, R.
AU - Freundlich, M.
PY - 1988/11
Y1 - 1988/11
N2 - Control of crp expression in vivo was studied by using the cloned crp gene. The synthesis of the product of this gene, cyclic AMP (cAMP) receptor protein (CRP), was strongly reduced by exogenous cAMP. This regulation was completely abolished by the inactivation of a divergent promoter located within the crp promoter region. These data are consistent with our in vitro studies (Okamoto and Freundlich, Proc.Natl. Acad. Sci. USA 83:5000-5004, 1986), which showed that crp autoregulation is due to the inhibition of crp transcription by divergent (antisense) RNA produced by cAMP-CRP activation of the divergent promoter.
AB - Control of crp expression in vivo was studied by using the cloned crp gene. The synthesis of the product of this gene, cyclic AMP (cAMP) receptor protein (CRP), was strongly reduced by exogenous cAMP. This regulation was completely abolished by the inactivation of a divergent promoter located within the crp promoter region. These data are consistent with our in vitro studies (Okamoto and Freundlich, Proc.Natl. Acad. Sci. USA 83:5000-5004, 1986), which showed that crp autoregulation is due to the inhibition of crp transcription by divergent (antisense) RNA produced by cAMP-CRP activation of the divergent promoter.
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U2 - 10.1128/jb.170.11.5076-5079.1988
DO - 10.1128/jb.170.11.5076-5079.1988
M3 - Article
C2 - 3053643
AN - SCOPUS:0024110098
SN - 0021-9193
VL - 170
SP - 5076
EP - 5079
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 11
ER -