Evidence for the presence of a novel biosynthetic pathway for norspermidine in Vibrio

S. Yamamoto, K. Hamanaka, Y. Suemoto, B. Ono, S. Shinoda

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Enzymatic studies of the cell extracts of Vibrio alginolyticus and V. parahaemolyticus provided evidence that there exists a novel biosynthetic pathway for norspermidine (NH2(CH2)3NH(CH2)3NH2), a major polyamine species. In this pathway, the Schiff base formed between aspartic β-semialdehyde and 1,3-diaminopropane is first reduced by a NADPH-dependent enzyme to yield 'carboxynorspermidine' (NH2(CH2)3NH(CH2)2CH(NH2)COOH), which is in turn decarboxylated by a pyridoxal phosphate dependent enzyme to form norspermidine. The end product and its intermediate were identified by gas chromatography-mass spectrometry. Experiments with L-[U-14C]aspartic acid resulted in appreciable incorporation of the label into norspermidine. Putrescine could replace 1,3-diaminopropane as a substrate to produce spermidine, but at a reduced rate. The enzyme activity was greatly enhanced by dithiothreitol. Since the activity of an aminopropyltransferase that utilizes decarboxylated S-adenosylmethionine as an aminopropyl group donor could not be detected in any of the cell extracts by our assay method, it was concluded that this novel pathway is primarily responsible for producing norspermidine and spermidine in these species.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalCanadian Journal of Microbiology
Volume32
Issue number2
Publication statusPublished - 1986
Externally publishedYes

Fingerprint

Vibrio
Biosynthetic Pathways
Spermidine
Cell Extracts
Spermidine Synthase
Enzymes
Vibrio alginolyticus
Pyridoxal Phosphate
Putrescine
Schiff Bases
Dithiothreitol
Polyamines
Enzyme activity
NADP
Aspartic Acid
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Labels
Assays

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Immunology
  • Microbiology

Cite this

Yamamoto, S., Hamanaka, K., Suemoto, Y., Ono, B., & Shinoda, S. (1986). Evidence for the presence of a novel biosynthetic pathway for norspermidine in Vibrio. Canadian Journal of Microbiology, 32(2), 99-103.

Evidence for the presence of a novel biosynthetic pathway for norspermidine in Vibrio. / Yamamoto, S.; Hamanaka, K.; Suemoto, Y.; Ono, B.; Shinoda, S.

In: Canadian Journal of Microbiology, Vol. 32, No. 2, 1986, p. 99-103.

Research output: Contribution to journalArticle

Yamamoto, S, Hamanaka, K, Suemoto, Y, Ono, B & Shinoda, S 1986, 'Evidence for the presence of a novel biosynthetic pathway for norspermidine in Vibrio', Canadian Journal of Microbiology, vol. 32, no. 2, pp. 99-103.
Yamamoto, S. ; Hamanaka, K. ; Suemoto, Y. ; Ono, B. ; Shinoda, S. / Evidence for the presence of a novel biosynthetic pathway for norspermidine in Vibrio. In: Canadian Journal of Microbiology. 1986 ; Vol. 32, No. 2. pp. 99-103.
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