Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1)

Mineo Yamaguchi, Takayuki Sasaki, Mayandi Sivaguru, Yoko Yamamoto, Hiroki Osawa, Ju Ahn Sung, Hideaki Matsumoto

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72 Citations (Scopus)

Abstract

Aluminum (Al)-activated malate transporter (ALMT1) was recently identified from wheat (Triticum aestivum). Heterologous expression of ALMT1 led to higher malate exudation that is associated with enhanced Al tolerance in transgenic plants. Here, we show the first direct evidence that ALMT1 is localized in the plasma membrane of Al-tolerant wheat. Phase partitioning experiments showed that this transporter was associated with the plasma membrane fraction. ALMT1 was detected in an Al-tolerant wheat line even without Al treatments. Analysis of transient expression of ALMT1::green fluorescent protein (GFP) in onion and tobacco cells further confirmed this ALMT1 localization.

Original languageEnglish
Pages (from-to)812-816
Number of pages5
JournalPlant and Cell Physiology
Volume46
Issue number5
DOIs
Publication statusPublished - May 1 2005

Keywords

  • Aluminum stress
  • Aluminum-activated malate transporter
  • Green fluorescent protein
  • Plasma membrane
  • Triticum aestivum L.

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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    Yamaguchi, M., Sasaki, T., Sivaguru, M., Yamamoto, Y., Osawa, H., Sung, J. A., & Matsumoto, H. (2005). Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1). Plant and Cell Physiology, 46(5), 812-816. https://doi.org/10.1093/pcp/pci083