Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1)

Mineo Yamaguchi, Takayuki Sasaki, Mayandi Sivaguru, Yoko Yamamoto, Hiroki Osawa, Ju Ahn Sung, Hideaki Matsumoto

    Research output: Contribution to journalArticlepeer-review

    76 Citations (Scopus)

    Abstract

    Aluminum (Al)-activated malate transporter (ALMT1) was recently identified from wheat (Triticum aestivum). Heterologous expression of ALMT1 led to higher malate exudation that is associated with enhanced Al tolerance in transgenic plants. Here, we show the first direct evidence that ALMT1 is localized in the plasma membrane of Al-tolerant wheat. Phase partitioning experiments showed that this transporter was associated with the plasma membrane fraction. ALMT1 was detected in an Al-tolerant wheat line even without Al treatments. Analysis of transient expression of ALMT1::green fluorescent protein (GFP) in onion and tobacco cells further confirmed this ALMT1 localization.

    Original languageEnglish
    Pages (from-to)812-816
    Number of pages5
    JournalPlant and Cell Physiology
    Volume46
    Issue number5
    DOIs
    Publication statusPublished - May 2005

    Keywords

    • Aluminum stress
    • Aluminum-activated malate transporter
    • Green fluorescent protein
    • Plasma membrane
    • Triticum aestivum L.

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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