Evidence for the lack of hepatic n-acetyltransferase in suncus (Suncus murinus)

Hironori Nakura, Susumu Itoh, Hisashi Kusano, Hiroyuki Ishizone, Takeo Deguchi, Tetsuya Kamataki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The abilities of liver cytosol fractions from the suncus and Sprague-Dawley (SD) rats to N-acetylate aniline, p-aminobenzoic acid, p-aminosalicylic acid and 2-aminofluorene (AF) were compared. The cytosol from rats N-acetylated these substrates at efficient rates, whereas the cytosol from the suncus did not N-acetylate these substrates at detectable rates. When AF was given to the suncus, 2-acetylaminofluorene (AAF), a metabolite of AF formed by N-acetyltransferase (NAT), was not detectable in serum, whereas the metabolite was seen clearly in rats. Northern blot and Southern blot analyses, using cDNAs coding for human NATs as probes, indicated that not only the transcripts but also the genes of the enzymes were undetectable in suncus. These results suggest that the suncus is among the few species known to lack NATs.

Original languageEnglish
Pages (from-to)1165-1170
Number of pages6
JournalBiochemical Pharmacology
Volume50
Issue number8
DOIs
Publication statusPublished - Oct 12 1995
Externally publishedYes

Fingerprint

Acetyltransferases
Cytosol
Rats
Metabolites
Liver
Aminosalicylic Acid
4-Aminobenzoic Acid
2-Acetylaminofluorene
Substrates
Southern Blotting
Northern Blotting
Sprague Dawley Rats
Complementary DNA
Genes
Enzymes
Serum
N-acetyltalosaminuronic acid

Keywords

  • 2-acetylaminofluorene
  • 2-aminofluorene
  • arylamine
  • liver, suncus
  • N-acetyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

Cite this

Evidence for the lack of hepatic n-acetyltransferase in suncus (Suncus murinus). / Nakura, Hironori; Itoh, Susumu; Kusano, Hisashi; Ishizone, Hiroyuki; Deguchi, Takeo; Kamataki, Tetsuya.

In: Biochemical Pharmacology, Vol. 50, No. 8, 12.10.1995, p. 1165-1170.

Research output: Contribution to journalArticle

Nakura, Hironori ; Itoh, Susumu ; Kusano, Hisashi ; Ishizone, Hiroyuki ; Deguchi, Takeo ; Kamataki, Tetsuya. / Evidence for the lack of hepatic n-acetyltransferase in suncus (Suncus murinus). In: Biochemical Pharmacology. 1995 ; Vol. 50, No. 8. pp. 1165-1170.
@article{c8e674281ca547bd84db462107dac852,
title = "Evidence for the lack of hepatic n-acetyltransferase in suncus (Suncus murinus)",
abstract = "The abilities of liver cytosol fractions from the suncus and Sprague-Dawley (SD) rats to N-acetylate aniline, p-aminobenzoic acid, p-aminosalicylic acid and 2-aminofluorene (AF) were compared. The cytosol from rats N-acetylated these substrates at efficient rates, whereas the cytosol from the suncus did not N-acetylate these substrates at detectable rates. When AF was given to the suncus, 2-acetylaminofluorene (AAF), a metabolite of AF formed by N-acetyltransferase (NAT), was not detectable in serum, whereas the metabolite was seen clearly in rats. Northern blot and Southern blot analyses, using cDNAs coding for human NATs as probes, indicated that not only the transcripts but also the genes of the enzymes were undetectable in suncus. These results suggest that the suncus is among the few species known to lack NATs.",
keywords = "2-acetylaminofluorene, 2-aminofluorene, arylamine, liver, suncus, N-acetyltransferase",
author = "Hironori Nakura and Susumu Itoh and Hisashi Kusano and Hiroyuki Ishizone and Takeo Deguchi and Tetsuya Kamataki",
year = "1995",
month = "10",
day = "12",
doi = "10.1016/0006-2952(95)00252-U",
language = "English",
volume = "50",
pages = "1165--1170",
journal = "Biochemical Pharmacology",
issn = "0006-2952",
publisher = "Elsevier Inc.",
number = "8",

}

TY - JOUR

T1 - Evidence for the lack of hepatic n-acetyltransferase in suncus (Suncus murinus)

AU - Nakura, Hironori

AU - Itoh, Susumu

AU - Kusano, Hisashi

AU - Ishizone, Hiroyuki

AU - Deguchi, Takeo

AU - Kamataki, Tetsuya

PY - 1995/10/12

Y1 - 1995/10/12

N2 - The abilities of liver cytosol fractions from the suncus and Sprague-Dawley (SD) rats to N-acetylate aniline, p-aminobenzoic acid, p-aminosalicylic acid and 2-aminofluorene (AF) were compared. The cytosol from rats N-acetylated these substrates at efficient rates, whereas the cytosol from the suncus did not N-acetylate these substrates at detectable rates. When AF was given to the suncus, 2-acetylaminofluorene (AAF), a metabolite of AF formed by N-acetyltransferase (NAT), was not detectable in serum, whereas the metabolite was seen clearly in rats. Northern blot and Southern blot analyses, using cDNAs coding for human NATs as probes, indicated that not only the transcripts but also the genes of the enzymes were undetectable in suncus. These results suggest that the suncus is among the few species known to lack NATs.

AB - The abilities of liver cytosol fractions from the suncus and Sprague-Dawley (SD) rats to N-acetylate aniline, p-aminobenzoic acid, p-aminosalicylic acid and 2-aminofluorene (AF) were compared. The cytosol from rats N-acetylated these substrates at efficient rates, whereas the cytosol from the suncus did not N-acetylate these substrates at detectable rates. When AF was given to the suncus, 2-acetylaminofluorene (AAF), a metabolite of AF formed by N-acetyltransferase (NAT), was not detectable in serum, whereas the metabolite was seen clearly in rats. Northern blot and Southern blot analyses, using cDNAs coding for human NATs as probes, indicated that not only the transcripts but also the genes of the enzymes were undetectable in suncus. These results suggest that the suncus is among the few species known to lack NATs.

KW - 2-acetylaminofluorene

KW - 2-aminofluorene

KW - arylamine

KW - liver, suncus

KW - N-acetyltransferase

UR - http://www.scopus.com/inward/record.url?scp=0028884102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028884102&partnerID=8YFLogxK

U2 - 10.1016/0006-2952(95)00252-U

DO - 10.1016/0006-2952(95)00252-U

M3 - Article

VL - 50

SP - 1165

EP - 1170

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

SN - 0006-2952

IS - 8

ER -