Evidence for the lack of hepatic n-acetyltransferase in suncus (Suncus murinus)

Hironori Nakura, Susumu Itoh, Hisashi Kusano, Hiroyuki Ishizone, Takeo Deguchi, Tetsuya Kamataki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The abilities of liver cytosol fractions from the suncus and Sprague-Dawley (SD) rats to N-acetylate aniline, p-aminobenzoic acid, p-aminosalicylic acid and 2-aminofluorene (AF) were compared. The cytosol from rats N-acetylated these substrates at efficient rates, whereas the cytosol from the suncus did not N-acetylate these substrates at detectable rates. When AF was given to the suncus, 2-acetylaminofluorene (AAF), a metabolite of AF formed by N-acetyltransferase (NAT), was not detectable in serum, whereas the metabolite was seen clearly in rats. Northern blot and Southern blot analyses, using cDNAs coding for human NATs as probes, indicated that not only the transcripts but also the genes of the enzymes were undetectable in suncus. These results suggest that the suncus is among the few species known to lack NATs.

Original languageEnglish
Pages (from-to)1165-1170
Number of pages6
JournalBiochemical Pharmacology
Volume50
Issue number8
DOIs
Publication statusPublished - Oct 12 1995
Externally publishedYes

Keywords

  • 2-acetylaminofluorene
  • 2-aminofluorene
  • N-acetyltransferase
  • arylamine
  • liver, suncus

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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