Evidence for new β1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera

Yoshinobu Kimura, Sho Sakamura, Takayuki Ushijima, Yoichiro Hama, Hiroyuki Kajiura, Kazuhito Fujiyama, Kiyoshi Okihara, Ken Hashimoto, Hiroyuki Sugimoto, Hideo Yamada

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7 Citations (Scopus)

Abstract

In a previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 70, 2583-2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Galβ1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new β1- 3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such β1-3galactosyltransferase activity, which can transfer galactosyl residues with the β1-3 linkage to β1-4 GalNAc residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAc 2GlcNAc2Man3-GlcNAc2-PA as acceptor N-glycan, we detected the β1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique β1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland.

Original languageEnglish
Pages (from-to)1111-1114
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number4
DOIs
Publication statusPublished - May 1 2007

Keywords

  • Apis mellifera
  • N-glycan
  • Royal jelly glycoprotein
  • Thomsen-Friedenreich antigen (Galβ1-3GalNAc)
  • β1-3 galactosyltransferase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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    Kimura, Y., Sakamura, S., Ushijima, T., Hama, Y., Kajiura, H., Fujiyama, K., Okihara, K., Hashimoto, K., Sugimoto, H., & Yamada, H. (2007). Evidence for new β1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera. Bioscience, Biotechnology and Biochemistry, 71(4), 1111-1114. https://doi.org/10.1271/bbb.70081