Evidence for new β1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera

Yoshinobu Kimura, Sho Sakamura, Takayuki Ushijima, Yoichiro Hama, Hiroyuki Kajiura, Kazuhito Fujiyama, Kiyoshi Okihara, Ken Hashimoto, Hiroyuki Sugimoto, Hideo Yamada

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In a previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 70, 2583-2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Galβ1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new β1- 3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such β1-3galactosyltransferase activity, which can transfer galactosyl residues with the β1-3 linkage to β1-4 GalNAc residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAc 2GlcNAc2Man3-GlcNAc2-PA as acceptor N-glycan, we detected the β1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique β1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland.

Original languageEnglish
Pages (from-to)1111-1114
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number4
DOIs
Publication statusPublished - 2007

Fingerprint

galactosyltransferases
Galactosyltransferases
Bees
Viral Tumor Antigens
Biosynthesis
Antigens
Apis mellifera
Polysaccharides
polysaccharides
biosynthesis
antigens
Glycoproteins
Tumors
honey bees
Membranes
royal jelly
Neoplasm Antigens
eukaryotic cells
glycoproteins
Head

Keywords

  • β1-3 galactosyltransferase
  • Apis mellifera
  • N-glycan
  • Royal jelly glycoprotein
  • Thomsen-Friedenreich antigen (Galβ1-3GalNAc)

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Evidence for new β1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera. / Kimura, Yoshinobu; Sakamura, Sho; Ushijima, Takayuki; Hama, Yoichiro; Kajiura, Hiroyuki; Fujiyama, Kazuhito; Okihara, Kiyoshi; Hashimoto, Ken; Sugimoto, Hiroyuki; Yamada, Hideo.

In: Bioscience, Biotechnology and Biochemistry, Vol. 71, No. 4, 2007, p. 1111-1114.

Research output: Contribution to journalArticle

Kimura, Y, Sakamura, S, Ushijima, T, Hama, Y, Kajiura, H, Fujiyama, K, Okihara, K, Hashimoto, K, Sugimoto, H & Yamada, H 2007, 'Evidence for new β1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera', Bioscience, Biotechnology and Biochemistry, vol. 71, no. 4, pp. 1111-1114. https://doi.org/10.1271/bbb.70081
Kimura, Yoshinobu ; Sakamura, Sho ; Ushijima, Takayuki ; Hama, Yoichiro ; Kajiura, Hiroyuki ; Fujiyama, Kazuhito ; Okihara, Kiyoshi ; Hashimoto, Ken ; Sugimoto, Hiroyuki ; Yamada, Hideo. / Evidence for new β1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera. In: Bioscience, Biotechnology and Biochemistry. 2007 ; Vol. 71, No. 4. pp. 1111-1114.
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