Evidence for involvement of mast cell degranulation and subsequent stimulation of histamine H1 and H2 receptors in radiographic contrast media-increased vascular permeability in rats

Takeshi Goromaru, Toshiaki Sendo, Yoshinori Itoh, Naoko Sakai, Daisuke Teshima, Ryozo Oishi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In the present study, the effects of systemic injection of radiographic contrast media (RCM) on vascular permeability was examined in various tissues of rats and the involvement of mast cell histamine in the RCM-induced vascular hyperpermeability subsequently determined. Both ionic (amidotrizoate) and non-ionic RCM (iohexol) enhanced vascular permeability specifically in lungs, as assessed by the extravasation of Evans blue (EB) dye. Another ionic RCM, ioxaglate, also markedly increased pulmonary vascular permeability and decreased pulmonary histamine content, whereby the extent of the reduction correlated well with the vascular hyperpermeability. Depletion of mast cells by prior treatment with compound 48/80 markedly attenuated the ioxaglate-induced enhancement of EB extravasation. The ioxaglate-increased extravasation was also inhibited by the mast cell stabilizer sodium cromoglicate and histamine H1 and H2 receptor antagonists. In isolated rat pulmonary mast cells, ioxaglate induced the concentration-dependent release of β-hexosaminidase, an index of mast cell degranulation. The present findings thus show clearly that RCM causes the degradation of pulmonary mast cells and the resultant release of histamine that in turn increases vascular permeability by stimulating histamine H1 and H2 receptors.

Original languageEnglish
Pages (from-to)605-612
Number of pages8
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume366
Issue number6
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Cell Degranulation
Histamine H1 Receptors
Histamine H2 Receptors
Capillary Permeability
Ioxaglic Acid
Mast Cells
Contrast Media
Lung
Evans Blue
Histamine
Blood Vessels
Histamine H1 Antagonists
Histamine H2 Antagonists
Iohexol
Diatrizoate
p-Methoxy-N-methylphenethylamine
Hexosaminidases
Histamine Release
Coloring Agents
Sodium

Keywords

  • Compound 48/80
  • H receptor antagonist
  • H receptor antagonist
  • Lung
  • Mast cell histamine
  • Radiographic contrast media
  • Vascular permeability

ASJC Scopus subject areas

  • Pharmacology

Cite this

Evidence for involvement of mast cell degranulation and subsequent stimulation of histamine H1 and H2 receptors in radiographic contrast media-increased vascular permeability in rats. / Goromaru, Takeshi; Sendo, Toshiaki; Itoh, Yoshinori; Sakai, Naoko; Teshima, Daisuke; Oishi, Ryozo.

In: Naunyn-Schmiedeberg's Archives of Pharmacology, Vol. 366, No. 6, 2002, p. 605-612.

Research output: Contribution to journalArticle

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