Evidence for estrogen receptor expression in the bursal epithelial cells of chicks

Young Ha Shin, Fumiko Takagi, Sachi Sugita, Song Han, Takehito Tsuji, Asaki Abe, Yasuhiro Kondo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of the present study was to demonstrate estrogen receptor (ER) α (ER-α) and ER-β expression in the bursa of Fabricius and cultured bursal epithelial cells (CBEC) by reverse transcription (RT)-polymerase chain reaction (PCR) using a primer set design based on the sequences of chicken ER-α complementary DNA (cDNA) and chicken ER-β-cDNA. In the case of ER-α, RT-PCR products of the expected size (698 base pairs (bp)) were amplified from cDNA derived from the bursa and CBEC, and from the oviduct, which was used as a positive control. All of the products were cleaved into two fragments of 476 bp and 222 bp by treating the products with restriction nuclease Hind III, indicating that these RT-PCR products from the bursa, CBEC and genital organs were the same. In contrast, in the case of ER-β, PCR products of the expected size (303 bp) were not detected in the bursa of Fabricius or CBEC, or in the oviduct and ovary. These results indicate that ER-α is expressed in the bursa of Fabricius and in CBEC, but ER-β is not expressed in the bursa or CBEC, or in the female genital organs of chickens.

Original languageEnglish
Pages (from-to)255-259
Number of pages5
JournalAnimal Science Journal
Volume76
Issue number3
Publication statusPublished - Jun 2005

Fingerprint

Estrogen Receptors
epithelial cells
chicks
Epithelial Cells
Bursa of Fabricius
Base Pairing
complementary DNA
bursa of Fabricius
Reverse Transcription
Chickens
Polymerase Chain Reaction
Oviducts
Complementary DNA
reverse transcriptase polymerase chain reaction
chickens
oviducts
genitalia
Female Genitalia
estrogen receptors
Genitalia

Keywords

  • Bursa of Fabricius
  • Chicken
  • Estrogen receptor α
  • Estrogen receptor β

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • veterinary(all)

Cite this

Shin, Y. H., Takagi, F., Sugita, S., Han, S., Tsuji, T., Abe, A., & Kondo, Y. (2005). Evidence for estrogen receptor expression in the bursal epithelial cells of chicks. Animal Science Journal, 76(3), 255-259.

Evidence for estrogen receptor expression in the bursal epithelial cells of chicks. / Shin, Young Ha; Takagi, Fumiko; Sugita, Sachi; Han, Song; Tsuji, Takehito; Abe, Asaki; Kondo, Yasuhiro.

In: Animal Science Journal, Vol. 76, No. 3, 06.2005, p. 255-259.

Research output: Contribution to journalArticle

Shin, YH, Takagi, F, Sugita, S, Han, S, Tsuji, T, Abe, A & Kondo, Y 2005, 'Evidence for estrogen receptor expression in the bursal epithelial cells of chicks', Animal Science Journal, vol. 76, no. 3, pp. 255-259.
Shin YH, Takagi F, Sugita S, Han S, Tsuji T, Abe A et al. Evidence for estrogen receptor expression in the bursal epithelial cells of chicks. Animal Science Journal. 2005 Jun;76(3):255-259.
Shin, Young Ha ; Takagi, Fumiko ; Sugita, Sachi ; Han, Song ; Tsuji, Takehito ; Abe, Asaki ; Kondo, Yasuhiro. / Evidence for estrogen receptor expression in the bursal epithelial cells of chicks. In: Animal Science Journal. 2005 ; Vol. 76, No. 3. pp. 255-259.
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