Abstract
The aim of the present study was to demonstrate estrogen receptor (ER) α (ER-α) and ER-β expression in the bursa of Fabricius and cultured bursal epithelial cells (CBEC) by reverse transcription (RT)-polymerase chain reaction (PCR) using a primer set design based on the sequences of chicken ER-α complementary DNA (cDNA) and chicken ER-β-cDNA. In the case of ER-α, RT-PCR products of the expected size (698 base pairs (bp)) were amplified from cDNA derived from the bursa and CBEC, and from the oviduct, which was used as a positive control. All of the products were cleaved into two fragments of 476 bp and 222 bp by treating the products with restriction nuclease Hind III, indicating that these RT-PCR products from the bursa, CBEC and genital organs were the same. In contrast, in the case of ER-β, PCR products of the expected size (303 bp) were not detected in the bursa of Fabricius or CBEC, or in the oviduct and ovary. These results indicate that ER-α is expressed in the bursa of Fabricius and in CBEC, but ER-β is not expressed in the bursa or CBEC, or in the female genital organs of chickens.
| Original language | English |
|---|---|
| Pages (from-to) | 255-259 |
| Number of pages | 5 |
| Journal | Animal Science Journal |
| Volume | 76 |
| Issue number | 3 |
| Publication status | Published - Jun 2005 |
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Keywords
- Bursa of Fabricius
- Chicken
- Estrogen receptor α
- Estrogen receptor β
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- veterinary(all)
Cite this
Evidence for estrogen receptor expression in the bursal epithelial cells of chicks. / Shin, Young Ha; Takagi, Fumiko; Sugita, Sachi; Han, Song; Tsuji, Takehito; Abe, Asaki; Kondo, Yasuhiro.
In: Animal Science Journal, Vol. 76, No. 3, 06.2005, p. 255-259.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence for estrogen receptor expression in the bursal epithelial cells of chicks
AU - Shin, Young Ha
AU - Takagi, Fumiko
AU - Sugita, Sachi
AU - Han, Song
AU - Tsuji, Takehito
AU - Abe, Asaki
AU - Kondo, Yasuhiro
PY - 2005/6
Y1 - 2005/6
N2 - The aim of the present study was to demonstrate estrogen receptor (ER) α (ER-α) and ER-β expression in the bursa of Fabricius and cultured bursal epithelial cells (CBEC) by reverse transcription (RT)-polymerase chain reaction (PCR) using a primer set design based on the sequences of chicken ER-α complementary DNA (cDNA) and chicken ER-β-cDNA. In the case of ER-α, RT-PCR products of the expected size (698 base pairs (bp)) were amplified from cDNA derived from the bursa and CBEC, and from the oviduct, which was used as a positive control. All of the products were cleaved into two fragments of 476 bp and 222 bp by treating the products with restriction nuclease Hind III, indicating that these RT-PCR products from the bursa, CBEC and genital organs were the same. In contrast, in the case of ER-β, PCR products of the expected size (303 bp) were not detected in the bursa of Fabricius or CBEC, or in the oviduct and ovary. These results indicate that ER-α is expressed in the bursa of Fabricius and in CBEC, but ER-β is not expressed in the bursa or CBEC, or in the female genital organs of chickens.
AB - The aim of the present study was to demonstrate estrogen receptor (ER) α (ER-α) and ER-β expression in the bursa of Fabricius and cultured bursal epithelial cells (CBEC) by reverse transcription (RT)-polymerase chain reaction (PCR) using a primer set design based on the sequences of chicken ER-α complementary DNA (cDNA) and chicken ER-β-cDNA. In the case of ER-α, RT-PCR products of the expected size (698 base pairs (bp)) were amplified from cDNA derived from the bursa and CBEC, and from the oviduct, which was used as a positive control. All of the products were cleaved into two fragments of 476 bp and 222 bp by treating the products with restriction nuclease Hind III, indicating that these RT-PCR products from the bursa, CBEC and genital organs were the same. In contrast, in the case of ER-β, PCR products of the expected size (303 bp) were not detected in the bursa of Fabricius or CBEC, or in the oviduct and ovary. These results indicate that ER-α is expressed in the bursa of Fabricius and in CBEC, but ER-β is not expressed in the bursa or CBEC, or in the female genital organs of chickens.
KW - Bursa of Fabricius
KW - Chicken
KW - Estrogen receptor α
KW - Estrogen receptor β
UR - http://www.scopus.com/inward/record.url?scp=22544480562&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22544480562&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:22544480562
VL - 76
SP - 255
EP - 259
JO - Animal Science Journal
JF - Animal Science Journal
SN - 1344-3941
IS - 3
ER -