TY - JOUR
T1 - Evidence for a PGF2α auto-amplification system in the endometrium in mares
AU - Kozai, Keisuke
AU - Tokuyama, Shota
AU - Szóstek, Anna Z.
AU - Toishi, Yuko
AU - Tsunoda, Nobuo
AU - Taya, Kazuyoshi
AU - Sakatani, Miki
AU - Takahashi, Masashi
AU - Nambo, Yasuo
AU - Skarzynski, Dariusz J.
AU - Yamamoto, Yuki
AU - Kimura, Koji
AU - Okuda, Kiyoshi
N1 - Funding Information:
This study was supported by the Japanese-Polish Joint Research Project under an agreement between the Japan Society for the Promotion of Science (JSPS) and Polish Academy of Sciences and was supported in part by the Equine Research Institute, Japan Racing Association. A Sz?stek was supported by Domestic Grants for Young Scientists from Foundation of Polish Science (FNP, Program Start, Poland) and a JSPS postdoctoral fellow (P-14082).
Publisher Copyright:
© 2016 Society for Reproduction and Fertility.
PY - 2016/5
Y1 - 2016/5
N2 - In mares, prostaglandin F2α (PGF2α) secreted from the endometrium is a major luteolysin. Some domestic animals have an autoamplification system in which PGF2α can stimulate its own production. Here, we investigated whether this is also the case in mares. In an in vivo study, mares at the mid-luteal phase (days 6-8 of estrous cycle) were injected i.m. with cloprostenol (250 ìg) and blood samples were collected at fixed intervals until 72 h after treatment. Progesterone (P4) concentrations started decreasing 45 min after the injection and continued to decrease up to 24 h (P < 0.05). In turn, 13,14-dihydro-15-keto-PGF2α (PGFM) metabolite started to increase 4h after an injection and continued to increase up to 72 h (P < 0.05). PGF receptor (PTGFR) mRNA expression in the endometrium was significantly higher in the late luteal phase than in the early and regressed luteal phases (P < 0.05). In vitro, PGF2α significantly stimulated (P < 0.05) PGF2α production by endometrial tissues and endometrial epithelial and stromal cells and significantly increased (P < 0.05) the mRNA expression of prostaglandin-endoperoxide synthase-2 (PTGS2), an enzyme involved in PGF2α synthesis in endometrial cell. These findings strongly suggest the existence of an endometrial PGF2α auto-amplification system in mares.
AB - In mares, prostaglandin F2α (PGF2α) secreted from the endometrium is a major luteolysin. Some domestic animals have an autoamplification system in which PGF2α can stimulate its own production. Here, we investigated whether this is also the case in mares. In an in vivo study, mares at the mid-luteal phase (days 6-8 of estrous cycle) were injected i.m. with cloprostenol (250 ìg) and blood samples were collected at fixed intervals until 72 h after treatment. Progesterone (P4) concentrations started decreasing 45 min after the injection and continued to decrease up to 24 h (P < 0.05). In turn, 13,14-dihydro-15-keto-PGF2α (PGFM) metabolite started to increase 4h after an injection and continued to increase up to 72 h (P < 0.05). PGF receptor (PTGFR) mRNA expression in the endometrium was significantly higher in the late luteal phase than in the early and regressed luteal phases (P < 0.05). In vitro, PGF2α significantly stimulated (P < 0.05) PGF2α production by endometrial tissues and endometrial epithelial and stromal cells and significantly increased (P < 0.05) the mRNA expression of prostaglandin-endoperoxide synthase-2 (PTGS2), an enzyme involved in PGF2α synthesis in endometrial cell. These findings strongly suggest the existence of an endometrial PGF2α auto-amplification system in mares.
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U2 - 10.1530/REP-15-0617
DO - 10.1530/REP-15-0617
M3 - Article
C2 - 26908917
AN - SCOPUS:84968920012
VL - 151
SP - 517
EP - 526
JO - Reviews of Reproduction
JF - Reviews of Reproduction
SN - 1470-1626
IS - 5
ER -