Evidence for a novel negative-stranded RNA mycovirus isolated from the plant pathogenic fungus Fusarium graminearum

Luan Wang, Hao He, Shuangchao Wang, Xiaoguang Chen, Dewen Qiu, Hideki Kondo, Lihua Guo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Here we describe a novel (−)ssRNA mycovirus, Fusarium graminearum negative-stranded RNA virus 1 (FgNSRV-1), isolated from Fusarium graminearum strain HN1. The genome of FgNSRV-1 is 9072 nucleotides in length, with five discontinuous but linear ORFs (ORF I-V). Phylogenetic analysis based on entire L polymerase sequences indicated that FgNSRV-1 is related to the (−)ssRNA mycovirus Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV-1), and other mycoviruses. Our data suggest that FgNSRV-1 can be classified into the family Mymonaviridae, order Mononegavirales. Putative enveloped virion-like structures with filamentous morphology similar to SsNSRV-1 were observed in virion preparation samples. The L proteins of FgNSRV-1, and other fungal mononegaviruses, were found to be related to L protein-like sequences in some fungal genome, supporting the hypothesis that there is coevolution occurring between mycoviruses and fungi. Besides, clearing the virus from the infected host fungus resulted in no discernable phenotypic change.

Original languageEnglish
Pages (from-to)232-240
Number of pages9
JournalVirology
Volume518
DOIs
Publication statusPublished - May 1 2018

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RNA Viruses
Fusarium
Fungi
RNA
Ascomycota
Virion
Open Reading Frames
Mononegavirales
Fungal Genome
Proteins
Nucleotides
Genome
Viruses

Keywords

  • Coevolution
  • FgNSRV-1
  • Fungus
  • Fusarium graminearum
  • Fusarium head blight
  • Mononegavirus
  • Mycovirus
  • Negative-stranded RNA virus

ASJC Scopus subject areas

  • Virology

Cite this

Evidence for a novel negative-stranded RNA mycovirus isolated from the plant pathogenic fungus Fusarium graminearum. / Wang, Luan; He, Hao; Wang, Shuangchao; Chen, Xiaoguang; Qiu, Dewen; Kondo, Hideki; Guo, Lihua.

In: Virology, Vol. 518, 01.05.2018, p. 232-240.

Research output: Contribution to journalArticle

Wang, Luan ; He, Hao ; Wang, Shuangchao ; Chen, Xiaoguang ; Qiu, Dewen ; Kondo, Hideki ; Guo, Lihua. / Evidence for a novel negative-stranded RNA mycovirus isolated from the plant pathogenic fungus Fusarium graminearum. In: Virology. 2018 ; Vol. 518. pp. 232-240.
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