Evaluation of virus resistance conferred by the NSs gene sequences from Tomato spotted wilt virus in transgenic plants

Shoji Sonoda, Masamichi Nishiguchi, Hisaaki Tsumuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Transgenic Nitcoticma benthamiana expressing the sense NSs gene sequence from Tomato spotted wilt virus (TSWV) was further transformed with the sense or antisense NSs gene sequence. Three sense and 10 antisense lines with two transformations (hereafter referred to as "double-transformed lines") that were expected to contain only the sense and both sense and antisense NSs gene sequences, respectively, were examined for homology-dependent virus resistance using the unrelated virus, Potato virus X containing the NSs gene sequence. As a result, it was shown that two antisense double-transformed lines contained plants that were symptom-free. Furthermore, both lines accumulated a small-interfering RNA. These results suggest that RNA silencing was operating in both lines. However, the lines exhibited little or no resistance to TSWV.

Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalBreeding Science
Volume55
Issue number1
DOIs
Publication statusPublished - Mar 2005

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Keywords

  • Nicotiana benthamiana
  • Nss gene
  • RNA silencing
  • Tomato spotted wilt virus
  • Virus resistance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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