TY - JOUR
T1 - Evaluation of urinary hydrogen peroxide as an oxidative stress biomarker in a healthy Japanese population
AU - Sato, Y.
AU - Ogino, K.
AU - Sakano, N.
AU - Wang, D. H.
AU - Yoshida, J.
AU - Akazawa, Y.
AU - Kanbara, S.
AU - Inoue, K.
AU - Kubo, M.
AU - Takahashi, H.
N1 - Funding Information:
The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper. This work was supported in part by funding from the Junpukai and the Health Science Center Foundation.
PY - 2013
Y1 - 2013
N2 - The usefulness of urinary hydrogen peroxide (H2O2) as an oxidative stress biomarker was evaluated in 766 healthy Japanese. The mean level of urinary concentrations of H2O2 was 5.66 ± 8.27 μmol/g creatinine, and was significantly higher in females than in males. Significant correlations of H2O2 were observed with age, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), insulin, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and exercise habit in females. In both sexes, H2O2 showed a significant correlation with 8-OHdG. By a multiple logistic regression analysis, urinary H2O2 was positively associated with urinary 8-OHdG and TC and was inversely associated with insulin. By stratification of sex and age, the association of urinary H2O2 with TC was positive in both sexes under 50 years old and was inverse in males over 50 years old, and that with insulin was inverse in males over 50 years old and in females under 50 years old. Moreover, by stratification of sex and age, a positive association of H2O2 with exercise and an inverse association of H2O2 with alcohol consumption became clear in males under 50 years old, although there were no significant odds for H2O2 after adjustment for covariates. In conclusion, the present results suggest that urinary H2O2 is a useful biomarker for oxidative stress, showing an association with 8-OHdG, TC, and insulin independently.
AB - The usefulness of urinary hydrogen peroxide (H2O2) as an oxidative stress biomarker was evaluated in 766 healthy Japanese. The mean level of urinary concentrations of H2O2 was 5.66 ± 8.27 μmol/g creatinine, and was significantly higher in females than in males. Significant correlations of H2O2 were observed with age, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), insulin, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and exercise habit in females. In both sexes, H2O2 showed a significant correlation with 8-OHdG. By a multiple logistic regression analysis, urinary H2O2 was positively associated with urinary 8-OHdG and TC and was inversely associated with insulin. By stratification of sex and age, the association of urinary H2O2 with TC was positive in both sexes under 50 years old and was inverse in males over 50 years old, and that with insulin was inverse in males over 50 years old and in females under 50 years old. Moreover, by stratification of sex and age, a positive association of H2O2 with exercise and an inverse association of H2O2 with alcohol consumption became clear in males under 50 years old, although there were no significant odds for H2O2 after adjustment for covariates. In conclusion, the present results suggest that urinary H2O2 is a useful biomarker for oxidative stress, showing an association with 8-OHdG, TC, and insulin independently.
KW - 8-OHdG
KW - Exercise
KW - H2O2
KW - Lifestyle
KW - Total cholesterol
UR - http://www.scopus.com/inward/record.url?scp=84873661559&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873661559&partnerID=8YFLogxK
U2 - 10.3109/10715762.2012.759218
DO - 10.3109/10715762.2012.759218
M3 - Article
C2 - 23249116
AN - SCOPUS:84873661559
VL - 47
SP - 181
EP - 191
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 3
ER -