Evaluation of urinary hydrogen peroxide as an oxidative stress biomarker in a healthy Japanese population

Y. Sato, Keiki Ogino, N. Sakano, D. H. Wang, J. Yoshida, Y. Akazawa, S. Kanbara, K. Inoue, M. Kubo, H. Takahashi

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Abstract

The usefulness of urinary hydrogen peroxide (H2O2) as an oxidative stress biomarker was evaluated in 766 healthy Japanese. The mean level of urinary concentrations of H2O2 was 5.66 ± 8.27 μmol/g creatinine, and was significantly higher in females than in males. Significant correlations of H2O2 were observed with age, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), insulin, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and exercise habit in females. In both sexes, H2O2 showed a significant correlation with 8-OHdG. By a multiple logistic regression analysis, urinary H2O2 was positively associated with urinary 8-OHdG and TC and was inversely associated with insulin. By stratification of sex and age, the association of urinary H2O2 with TC was positive in both sexes under 50 years old and was inverse in males over 50 years old, and that with insulin was inverse in males over 50 years old and in females under 50 years old. Moreover, by stratification of sex and age, a positive association of H2O2 with exercise and an inverse association of H2O2 with alcohol consumption became clear in males under 50 years old, although there were no significant odds for H2O2 after adjustment for covariates. In conclusion, the present results suggest that urinary H2O2 is a useful biomarker for oxidative stress, showing an association with 8-OHdG, TC, and insulin independently.

Original languageEnglish
Pages (from-to)181-191
Number of pages11
JournalFree Radical Research
Volume47
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

Oxidative stress
Biomarkers
Hydrogen Peroxide
Oxidative Stress
Cholesterol
Association reactions
Insulin
Population
Exercise
Aspartate Aminotransferases
Alanine Transaminase
Regression analysis
Alcohol Drinking
LDL Cholesterol
Habits
Logistics
Creatinine
Logistic Models
Regression Analysis
Alcohols

Keywords

  • 8-OHdG
  • Exercise
  • H2O2
  • Lifestyle
  • Total cholesterol

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sato, Y., Ogino, K., Sakano, N., Wang, D. H., Yoshida, J., Akazawa, Y., ... Takahashi, H. (2013). Evaluation of urinary hydrogen peroxide as an oxidative stress biomarker in a healthy Japanese population. Free Radical Research, 47(3), 181-191. https://doi.org/10.3109/10715762.2012.759218

Evaluation of urinary hydrogen peroxide as an oxidative stress biomarker in a healthy Japanese population. / Sato, Y.; Ogino, Keiki; Sakano, N.; Wang, D. H.; Yoshida, J.; Akazawa, Y.; Kanbara, S.; Inoue, K.; Kubo, M.; Takahashi, H.

In: Free Radical Research, Vol. 47, No. 3, 2013, p. 181-191.

Research output: Contribution to journalArticle

Sato, Y, Ogino, K, Sakano, N, Wang, DH, Yoshida, J, Akazawa, Y, Kanbara, S, Inoue, K, Kubo, M & Takahashi, H 2013, 'Evaluation of urinary hydrogen peroxide as an oxidative stress biomarker in a healthy Japanese population', Free Radical Research, vol. 47, no. 3, pp. 181-191. https://doi.org/10.3109/10715762.2012.759218
Sato, Y. ; Ogino, Keiki ; Sakano, N. ; Wang, D. H. ; Yoshida, J. ; Akazawa, Y. ; Kanbara, S. ; Inoue, K. ; Kubo, M. ; Takahashi, H. / Evaluation of urinary hydrogen peroxide as an oxidative stress biomarker in a healthy Japanese population. In: Free Radical Research. 2013 ; Vol. 47, No. 3. pp. 181-191.
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