Evaluation of the reproductive and developmental toxicity of aluminium ammonium sulfate in a two-generation study in rats

Mutsuko Hirata-Koizumi, Sakiko Fujii, Atsushi Ono, Akihiko Hirose, Toshio Imai, Kumiko Ogawa, Makoto Ema, Akiyoshi Nishikawa

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Aluminium ammonium sulfate (AAS) was tested for reproductive/developmental toxicity in a two-generation study. Male and female rats were continuously given AAS in drinking water at 0, 50, 500 or 5000. ppm. Water consumption was decreased in all AAS-treated groups, and the body weight of parental animals transiently decreased in the 5000. ppm group. In either generation, no compound-related changes were found in estrous cyclicity, sperm parameters, copulation, fertility and gestation index, number of implantations and live birth pups, sex ratios of pups or viability during the preweaning period. Male and female F1 pups in the 5000. ppm group showed a lower body weight on postnatal day 21, while there were no differences in the birth weight of F1 and F2 pups between the control and AAS-treated groups. Preweaning body weight gain in F2 males and females indicated a similar decreasing tendency at 5000. ppm. In F1 and F2 weanlings, the weight of the liver, spleen and thymus decreased at 5000. ppm, but no histopathological changes were found in these organs. In F1 females in the 5000. ppm group, vaginal opening was delayed slightly. There were no compound-related changes in male preputial separation or in other developmental landmarks. In behavioral tests conducted for F1 animals at 4-6. weeks of age, no compound-related changes were found in spontaneous locomotor activity and performance in a water-filled multiple T-maze. In conclusion, the NOAEL of AAS for two-generation reproductive/developmental toxicity was considered to be 500. ppm in rats. Considering the aluminium content in the basal diet, the total ingested dose of aluminium from drinking water and food in this 500. ppm group was calculated to be 5.35. mg. Al/kg. bw/day.

Original languageEnglish
Pages (from-to)1948-1959
Number of pages12
JournalFood and Chemical Toxicology
Volume49
Issue number9
DOIs
Publication statusPublished - Sep 2011
Externally publishedYes

Fingerprint

reproductive toxicology
developmental toxicity
aluminum sulfate
ammonium sulfate
Toxicity
Rats
pups
rats
Body Weight
Aluminum
Drinking Water
drinking water
body weight
aluminum
Animals
No-Observed-Adverse-Effect Level
Thymus
Copulation
no observed adverse effect level
Water

Keywords

  • Aluminium ammonium sulfate
  • Developmental toxicity
  • Food additive
  • Rat
  • Two-generation reproductive toxicity

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Evaluation of the reproductive and developmental toxicity of aluminium ammonium sulfate in a two-generation study in rats. / Hirata-Koizumi, Mutsuko; Fujii, Sakiko; Ono, Atsushi; Hirose, Akihiko; Imai, Toshio; Ogawa, Kumiko; Ema, Makoto; Nishikawa, Akiyoshi.

In: Food and Chemical Toxicology, Vol. 49, No. 9, 09.2011, p. 1948-1959.

Research output: Contribution to journalArticle

Hirata-Koizumi, Mutsuko ; Fujii, Sakiko ; Ono, Atsushi ; Hirose, Akihiko ; Imai, Toshio ; Ogawa, Kumiko ; Ema, Makoto ; Nishikawa, Akiyoshi. / Evaluation of the reproductive and developmental toxicity of aluminium ammonium sulfate in a two-generation study in rats. In: Food and Chemical Toxicology. 2011 ; Vol. 49, No. 9. pp. 1948-1959.
@article{bcd4f070d4424ee382acc270c25dae87,
title = "Evaluation of the reproductive and developmental toxicity of aluminium ammonium sulfate in a two-generation study in rats",
abstract = "Aluminium ammonium sulfate (AAS) was tested for reproductive/developmental toxicity in a two-generation study. Male and female rats were continuously given AAS in drinking water at 0, 50, 500 or 5000. ppm. Water consumption was decreased in all AAS-treated groups, and the body weight of parental animals transiently decreased in the 5000. ppm group. In either generation, no compound-related changes were found in estrous cyclicity, sperm parameters, copulation, fertility and gestation index, number of implantations and live birth pups, sex ratios of pups or viability during the preweaning period. Male and female F1 pups in the 5000. ppm group showed a lower body weight on postnatal day 21, while there were no differences in the birth weight of F1 and F2 pups between the control and AAS-treated groups. Preweaning body weight gain in F2 males and females indicated a similar decreasing tendency at 5000. ppm. In F1 and F2 weanlings, the weight of the liver, spleen and thymus decreased at 5000. ppm, but no histopathological changes were found in these organs. In F1 females in the 5000. ppm group, vaginal opening was delayed slightly. There were no compound-related changes in male preputial separation or in other developmental landmarks. In behavioral tests conducted for F1 animals at 4-6. weeks of age, no compound-related changes were found in spontaneous locomotor activity and performance in a water-filled multiple T-maze. In conclusion, the NOAEL of AAS for two-generation reproductive/developmental toxicity was considered to be 500. ppm in rats. Considering the aluminium content in the basal diet, the total ingested dose of aluminium from drinking water and food in this 500. ppm group was calculated to be 5.35. mg. Al/kg. bw/day.",
keywords = "Aluminium ammonium sulfate, Developmental toxicity, Food additive, Rat, Two-generation reproductive toxicity",
author = "Mutsuko Hirata-Koizumi and Sakiko Fujii and Atsushi Ono and Akihiko Hirose and Toshio Imai and Kumiko Ogawa and Makoto Ema and Akiyoshi Nishikawa",
year = "2011",
month = "9",
doi = "10.1016/j.fct.2011.04.035",
language = "English",
volume = "49",
pages = "1948--1959",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Elsevier Limited",
number = "9",

}

TY - JOUR

T1 - Evaluation of the reproductive and developmental toxicity of aluminium ammonium sulfate in a two-generation study in rats

AU - Hirata-Koizumi, Mutsuko

AU - Fujii, Sakiko

AU - Ono, Atsushi

AU - Hirose, Akihiko

AU - Imai, Toshio

AU - Ogawa, Kumiko

AU - Ema, Makoto

AU - Nishikawa, Akiyoshi

PY - 2011/9

Y1 - 2011/9

N2 - Aluminium ammonium sulfate (AAS) was tested for reproductive/developmental toxicity in a two-generation study. Male and female rats were continuously given AAS in drinking water at 0, 50, 500 or 5000. ppm. Water consumption was decreased in all AAS-treated groups, and the body weight of parental animals transiently decreased in the 5000. ppm group. In either generation, no compound-related changes were found in estrous cyclicity, sperm parameters, copulation, fertility and gestation index, number of implantations and live birth pups, sex ratios of pups or viability during the preweaning period. Male and female F1 pups in the 5000. ppm group showed a lower body weight on postnatal day 21, while there were no differences in the birth weight of F1 and F2 pups between the control and AAS-treated groups. Preweaning body weight gain in F2 males and females indicated a similar decreasing tendency at 5000. ppm. In F1 and F2 weanlings, the weight of the liver, spleen and thymus decreased at 5000. ppm, but no histopathological changes were found in these organs. In F1 females in the 5000. ppm group, vaginal opening was delayed slightly. There were no compound-related changes in male preputial separation or in other developmental landmarks. In behavioral tests conducted for F1 animals at 4-6. weeks of age, no compound-related changes were found in spontaneous locomotor activity and performance in a water-filled multiple T-maze. In conclusion, the NOAEL of AAS for two-generation reproductive/developmental toxicity was considered to be 500. ppm in rats. Considering the aluminium content in the basal diet, the total ingested dose of aluminium from drinking water and food in this 500. ppm group was calculated to be 5.35. mg. Al/kg. bw/day.

AB - Aluminium ammonium sulfate (AAS) was tested for reproductive/developmental toxicity in a two-generation study. Male and female rats were continuously given AAS in drinking water at 0, 50, 500 or 5000. ppm. Water consumption was decreased in all AAS-treated groups, and the body weight of parental animals transiently decreased in the 5000. ppm group. In either generation, no compound-related changes were found in estrous cyclicity, sperm parameters, copulation, fertility and gestation index, number of implantations and live birth pups, sex ratios of pups or viability during the preweaning period. Male and female F1 pups in the 5000. ppm group showed a lower body weight on postnatal day 21, while there were no differences in the birth weight of F1 and F2 pups between the control and AAS-treated groups. Preweaning body weight gain in F2 males and females indicated a similar decreasing tendency at 5000. ppm. In F1 and F2 weanlings, the weight of the liver, spleen and thymus decreased at 5000. ppm, but no histopathological changes were found in these organs. In F1 females in the 5000. ppm group, vaginal opening was delayed slightly. There were no compound-related changes in male preputial separation or in other developmental landmarks. In behavioral tests conducted for F1 animals at 4-6. weeks of age, no compound-related changes were found in spontaneous locomotor activity and performance in a water-filled multiple T-maze. In conclusion, the NOAEL of AAS for two-generation reproductive/developmental toxicity was considered to be 500. ppm in rats. Considering the aluminium content in the basal diet, the total ingested dose of aluminium from drinking water and food in this 500. ppm group was calculated to be 5.35. mg. Al/kg. bw/day.

KW - Aluminium ammonium sulfate

KW - Developmental toxicity

KW - Food additive

KW - Rat

KW - Two-generation reproductive toxicity

UR - http://www.scopus.com/inward/record.url?scp=79960844657&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960844657&partnerID=8YFLogxK

U2 - 10.1016/j.fct.2011.04.035

DO - 10.1016/j.fct.2011.04.035

M3 - Article

VL - 49

SP - 1948

EP - 1959

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

IS - 9

ER -