Abstract
Aluminium ammonium sulfate (AAS) was tested for reproductive/developmental toxicity in a two-generation study. Male and female rats were continuously given AAS in drinking water at 0, 50, 500 or 5000. ppm. Water consumption was decreased in all AAS-treated groups, and the body weight of parental animals transiently decreased in the 5000. ppm group. In either generation, no compound-related changes were found in estrous cyclicity, sperm parameters, copulation, fertility and gestation index, number of implantations and live birth pups, sex ratios of pups or viability during the preweaning period. Male and female F1 pups in the 5000. ppm group showed a lower body weight on postnatal day 21, while there were no differences in the birth weight of F1 and F2 pups between the control and AAS-treated groups. Preweaning body weight gain in F2 males and females indicated a similar decreasing tendency at 5000. ppm. In F1 and F2 weanlings, the weight of the liver, spleen and thymus decreased at 5000. ppm, but no histopathological changes were found in these organs. In F1 females in the 5000. ppm group, vaginal opening was delayed slightly. There were no compound-related changes in male preputial separation or in other developmental landmarks. In behavioral tests conducted for F1 animals at 4-6. weeks of age, no compound-related changes were found in spontaneous locomotor activity and performance in a water-filled multiple T-maze. In conclusion, the NOAEL of AAS for two-generation reproductive/developmental toxicity was considered to be 500. ppm in rats. Considering the aluminium content in the basal diet, the total ingested dose of aluminium from drinking water and food in this 500. ppm group was calculated to be 5.35. mg. Al/kg. bw/day.
| Original language | English |
|---|---|
| Pages (from-to) | 1948-1959 |
| Number of pages | 12 |
| Journal | Food and Chemical Toxicology |
| Volume | 49 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 2011 |
| Externally published | Yes |
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Keywords
- Aluminium ammonium sulfate
- Developmental toxicity
- Food additive
- Rat
- Two-generation reproductive toxicity
ASJC Scopus subject areas
- Food Science
- Toxicology
Cite this
Evaluation of the reproductive and developmental toxicity of aluminium ammonium sulfate in a two-generation study in rats. / Hirata-Koizumi, Mutsuko; Fujii, Sakiko; Ono, Atsushi; Hirose, Akihiko; Imai, Toshio; Ogawa, Kumiko; Ema, Makoto; Nishikawa, Akiyoshi.
In: Food and Chemical Toxicology, Vol. 49, No. 9, 09.2011, p. 1948-1959.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of the reproductive and developmental toxicity of aluminium ammonium sulfate in a two-generation study in rats
AU - Hirata-Koizumi, Mutsuko
AU - Fujii, Sakiko
AU - Ono, Atsushi
AU - Hirose, Akihiko
AU - Imai, Toshio
AU - Ogawa, Kumiko
AU - Ema, Makoto
AU - Nishikawa, Akiyoshi
PY - 2011/9
Y1 - 2011/9
N2 - Aluminium ammonium sulfate (AAS) was tested for reproductive/developmental toxicity in a two-generation study. Male and female rats were continuously given AAS in drinking water at 0, 50, 500 or 5000. ppm. Water consumption was decreased in all AAS-treated groups, and the body weight of parental animals transiently decreased in the 5000. ppm group. In either generation, no compound-related changes were found in estrous cyclicity, sperm parameters, copulation, fertility and gestation index, number of implantations and live birth pups, sex ratios of pups or viability during the preweaning period. Male and female F1 pups in the 5000. ppm group showed a lower body weight on postnatal day 21, while there were no differences in the birth weight of F1 and F2 pups between the control and AAS-treated groups. Preweaning body weight gain in F2 males and females indicated a similar decreasing tendency at 5000. ppm. In F1 and F2 weanlings, the weight of the liver, spleen and thymus decreased at 5000. ppm, but no histopathological changes were found in these organs. In F1 females in the 5000. ppm group, vaginal opening was delayed slightly. There were no compound-related changes in male preputial separation or in other developmental landmarks. In behavioral tests conducted for F1 animals at 4-6. weeks of age, no compound-related changes were found in spontaneous locomotor activity and performance in a water-filled multiple T-maze. In conclusion, the NOAEL of AAS for two-generation reproductive/developmental toxicity was considered to be 500. ppm in rats. Considering the aluminium content in the basal diet, the total ingested dose of aluminium from drinking water and food in this 500. ppm group was calculated to be 5.35. mg. Al/kg. bw/day.
AB - Aluminium ammonium sulfate (AAS) was tested for reproductive/developmental toxicity in a two-generation study. Male and female rats were continuously given AAS in drinking water at 0, 50, 500 or 5000. ppm. Water consumption was decreased in all AAS-treated groups, and the body weight of parental animals transiently decreased in the 5000. ppm group. In either generation, no compound-related changes were found in estrous cyclicity, sperm parameters, copulation, fertility and gestation index, number of implantations and live birth pups, sex ratios of pups or viability during the preweaning period. Male and female F1 pups in the 5000. ppm group showed a lower body weight on postnatal day 21, while there were no differences in the birth weight of F1 and F2 pups between the control and AAS-treated groups. Preweaning body weight gain in F2 males and females indicated a similar decreasing tendency at 5000. ppm. In F1 and F2 weanlings, the weight of the liver, spleen and thymus decreased at 5000. ppm, but no histopathological changes were found in these organs. In F1 females in the 5000. ppm group, vaginal opening was delayed slightly. There were no compound-related changes in male preputial separation or in other developmental landmarks. In behavioral tests conducted for F1 animals at 4-6. weeks of age, no compound-related changes were found in spontaneous locomotor activity and performance in a water-filled multiple T-maze. In conclusion, the NOAEL of AAS for two-generation reproductive/developmental toxicity was considered to be 500. ppm in rats. Considering the aluminium content in the basal diet, the total ingested dose of aluminium from drinking water and food in this 500. ppm group was calculated to be 5.35. mg. Al/kg. bw/day.
KW - Aluminium ammonium sulfate
KW - Developmental toxicity
KW - Food additive
KW - Rat
KW - Two-generation reproductive toxicity
UR - http://www.scopus.com/inward/record.url?scp=79960844657&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960844657&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2011.04.035
DO - 10.1016/j.fct.2011.04.035
M3 - Article
VL - 49
SP - 1948
EP - 1959
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
SN - 0278-6915
IS - 9
ER -