Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance

Eriko Aoyama, Masaharu Takigawa

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

CCN proteins are known to bind to various growth factors, cytokines, and membrane proteins. Since these bindings are closely involved in the function of CCN proteins, the analysis of the binding partners is the first step toward understanding the mechanisms of actions of CCN proteins. This chapter describes two approaches used for such analyses: a solid-phase binding assay, which is suitable for confirming the binding easily because of its simplicity and cost advantage, and a surface plasmon resonance assay, which can determine the binding affinities between CCN proteins and their partners.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages77-86
Number of pages10
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2582
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • binding proteins
  • CCN2
  • peptide
  • solid-phase binding assay
  • SPR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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