Evaluation of the dynamics of choroidal circulation in experimental acute hypertension using indocyanine green-stained leukocytes

Toshio Okanouchi, Fumio Shiraga, Ippei Takasu, Yozo Tsuchida, Hiroshi Ohtsuki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: To evaluate the dynamics of choroidal circulation in experimental acute hypertension, using the indocyanine green leukocyte angiography (ILA) method, which the authors have developed for the evaluation of leukocyte dynamics in choroidal circulation. Methods: Japan White rabbits were used in the present study. Leukocytes were collected by centrifugal separation of the autologous blood, and were stained with indocyanine green (ICG) dye. The ICG-stained leukocyte fluid was injected into an ear vein, and fundus images were obtained by infrared laser and a scanning laser ophthalmoscope. Experimental acute hypertension was induced by the intravenous drip injection of angiotensin II (AII). Results: The fluorescent dots rapidly moved in choroidal arteries at a decreasing velocity, passed very slowly through choroidal capillaries and drained into choroidal veins. Under normal blood pressure, the mean leukocyte velocities in arteries, capillaries and veins were 8.63±1.68, 0.52±0.07, and 6.96±2.20 mm/s, respectively. On the other hand, the respective mean velocities in acute hypertension induced by AII were 13.50±1.82, 0.81±0.09, and 10.54±3.91 mm/s. Besides flow velocity, no change in leukocyte dynamics was observed. Conclusions: Under the condition of acute hypertension induced by AII, leukocytes moved faster in the total choroidal circulation (from arteries to veins) compared to their velocity under the condition of normal blood pressure. Blood velocities might increase in the total choroidal circulation at an early stage in acute hypertension induced by AII, resulting in increased choroidal blood flow. ILA makes it possible to evaluate the changes in choroidal circulation under various pathologic conditions.

Original languageEnglish
Pages (from-to)572-577
Number of pages6
JournalJapanese Journal of Ophthalmology
Volume47
Issue number6
DOIs
Publication statusPublished - Nov 2003
Externally publishedYes

Fingerprint

Indocyanine Green
Leukocytes
Hypertension
Angiotensin II
Veins
Arteries
Angiography
Lasers
Blood Pressure
Ophthalmoscopes
Intravenous Infusions
Intravenous Injections
Ear
Japan
Coloring Agents
Rabbits

Keywords

  • Angiotensin II
  • Choroidal circulation
  • Hypertension
  • Indocyanine green angiography
  • Leukocyte

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Evaluation of the dynamics of choroidal circulation in experimental acute hypertension using indocyanine green-stained leukocytes. / Okanouchi, Toshio; Shiraga, Fumio; Takasu, Ippei; Tsuchida, Yozo; Ohtsuki, Hiroshi.

In: Japanese Journal of Ophthalmology, Vol. 47, No. 6, 11.2003, p. 572-577.

Research output: Contribution to journalArticle

@article{c5d7d79a0bbe44ab8aa95398dde26f43,
title = "Evaluation of the dynamics of choroidal circulation in experimental acute hypertension using indocyanine green-stained leukocytes",
abstract = "Purpose: To evaluate the dynamics of choroidal circulation in experimental acute hypertension, using the indocyanine green leukocyte angiography (ILA) method, which the authors have developed for the evaluation of leukocyte dynamics in choroidal circulation. Methods: Japan White rabbits were used in the present study. Leukocytes were collected by centrifugal separation of the autologous blood, and were stained with indocyanine green (ICG) dye. The ICG-stained leukocyte fluid was injected into an ear vein, and fundus images were obtained by infrared laser and a scanning laser ophthalmoscope. Experimental acute hypertension was induced by the intravenous drip injection of angiotensin II (AII). Results: The fluorescent dots rapidly moved in choroidal arteries at a decreasing velocity, passed very slowly through choroidal capillaries and drained into choroidal veins. Under normal blood pressure, the mean leukocyte velocities in arteries, capillaries and veins were 8.63±1.68, 0.52±0.07, and 6.96±2.20 mm/s, respectively. On the other hand, the respective mean velocities in acute hypertension induced by AII were 13.50±1.82, 0.81±0.09, and 10.54±3.91 mm/s. Besides flow velocity, no change in leukocyte dynamics was observed. Conclusions: Under the condition of acute hypertension induced by AII, leukocytes moved faster in the total choroidal circulation (from arteries to veins) compared to their velocity under the condition of normal blood pressure. Blood velocities might increase in the total choroidal circulation at an early stage in acute hypertension induced by AII, resulting in increased choroidal blood flow. ILA makes it possible to evaluate the changes in choroidal circulation under various pathologic conditions.",
keywords = "Angiotensin II, Choroidal circulation, Hypertension, Indocyanine green angiography, Leukocyte",
author = "Toshio Okanouchi and Fumio Shiraga and Ippei Takasu and Yozo Tsuchida and Hiroshi Ohtsuki",
year = "2003",
month = "11",
doi = "10.1016/S0021-5155(03)00147-3",
language = "English",
volume = "47",
pages = "572--577",
journal = "Japanese Journal of Ophthalmology",
issn = "0021-5155",
publisher = "Springer Japan",
number = "6",

}

TY - JOUR

T1 - Evaluation of the dynamics of choroidal circulation in experimental acute hypertension using indocyanine green-stained leukocytes

AU - Okanouchi, Toshio

AU - Shiraga, Fumio

AU - Takasu, Ippei

AU - Tsuchida, Yozo

AU - Ohtsuki, Hiroshi

PY - 2003/11

Y1 - 2003/11

N2 - Purpose: To evaluate the dynamics of choroidal circulation in experimental acute hypertension, using the indocyanine green leukocyte angiography (ILA) method, which the authors have developed for the evaluation of leukocyte dynamics in choroidal circulation. Methods: Japan White rabbits were used in the present study. Leukocytes were collected by centrifugal separation of the autologous blood, and were stained with indocyanine green (ICG) dye. The ICG-stained leukocyte fluid was injected into an ear vein, and fundus images were obtained by infrared laser and a scanning laser ophthalmoscope. Experimental acute hypertension was induced by the intravenous drip injection of angiotensin II (AII). Results: The fluorescent dots rapidly moved in choroidal arteries at a decreasing velocity, passed very slowly through choroidal capillaries and drained into choroidal veins. Under normal blood pressure, the mean leukocyte velocities in arteries, capillaries and veins were 8.63±1.68, 0.52±0.07, and 6.96±2.20 mm/s, respectively. On the other hand, the respective mean velocities in acute hypertension induced by AII were 13.50±1.82, 0.81±0.09, and 10.54±3.91 mm/s. Besides flow velocity, no change in leukocyte dynamics was observed. Conclusions: Under the condition of acute hypertension induced by AII, leukocytes moved faster in the total choroidal circulation (from arteries to veins) compared to their velocity under the condition of normal blood pressure. Blood velocities might increase in the total choroidal circulation at an early stage in acute hypertension induced by AII, resulting in increased choroidal blood flow. ILA makes it possible to evaluate the changes in choroidal circulation under various pathologic conditions.

AB - Purpose: To evaluate the dynamics of choroidal circulation in experimental acute hypertension, using the indocyanine green leukocyte angiography (ILA) method, which the authors have developed for the evaluation of leukocyte dynamics in choroidal circulation. Methods: Japan White rabbits were used in the present study. Leukocytes were collected by centrifugal separation of the autologous blood, and were stained with indocyanine green (ICG) dye. The ICG-stained leukocyte fluid was injected into an ear vein, and fundus images were obtained by infrared laser and a scanning laser ophthalmoscope. Experimental acute hypertension was induced by the intravenous drip injection of angiotensin II (AII). Results: The fluorescent dots rapidly moved in choroidal arteries at a decreasing velocity, passed very slowly through choroidal capillaries and drained into choroidal veins. Under normal blood pressure, the mean leukocyte velocities in arteries, capillaries and veins were 8.63±1.68, 0.52±0.07, and 6.96±2.20 mm/s, respectively. On the other hand, the respective mean velocities in acute hypertension induced by AII were 13.50±1.82, 0.81±0.09, and 10.54±3.91 mm/s. Besides flow velocity, no change in leukocyte dynamics was observed. Conclusions: Under the condition of acute hypertension induced by AII, leukocytes moved faster in the total choroidal circulation (from arteries to veins) compared to their velocity under the condition of normal blood pressure. Blood velocities might increase in the total choroidal circulation at an early stage in acute hypertension induced by AII, resulting in increased choroidal blood flow. ILA makes it possible to evaluate the changes in choroidal circulation under various pathologic conditions.

KW - Angiotensin II

KW - Choroidal circulation

KW - Hypertension

KW - Indocyanine green angiography

KW - Leukocyte

UR - http://www.scopus.com/inward/record.url?scp=0242720381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242720381&partnerID=8YFLogxK

U2 - 10.1016/S0021-5155(03)00147-3

DO - 10.1016/S0021-5155(03)00147-3

M3 - Article

VL - 47

SP - 572

EP - 577

JO - Japanese Journal of Ophthalmology

JF - Japanese Journal of Ophthalmology

SN - 0021-5155

IS - 6

ER -