Evaluation of the biofilm-forming ability and genetic typing for clinical isolates of Pseudomonas aeruginosa by enterobacterial repetitive intergenic consensus-based PCR

Weiqing Yang, Lei Shi, Wen Xiang Jia, Xiaolin Yin, Jian Yu Su, Yali Kou, Xie Yi, Sumio Shinoda, Shin Ichi Miyoshi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Biofilm formation is an important phenotype associated with chronic Pseudomonas aeruginosa infections. In the present study, a total of 48 P. aeruginosa strains isolated from clinical specimens were examined for their biofilm-forming ability using a microtiter plate method. The different biofilm-forming abilities were demonstrated among the strains; however, most strains formed a larger biofilm than strain PAO1, a reference strain. The genetic typing was also carried out by enterobacterial repetitive intergenic consensus-based polymerase chain reaction. Although they were divided into five groups (A to E), most of the strains showing the higher biofilm-forming ability were found to be in groups D and E, suggesting a significant relationship between the biofilm-forming ability and the genetic group.

Original languageEnglish
Pages (from-to)1057-1061
Number of pages5
JournalMICROBIOLOGY and IMMUNOLOGY
Volume49
Issue number12
DOIs
Publication statusPublished - Jan 1 2005

Keywords

  • Biofilm
  • Genetic typing
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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