Evaluation of the biofilm-forming ability and genetic typing for clinical isolates of Pseudomonas aeruginosa by enterobacterial repetitive intergenic consensus-based PCR

Weiqing Yang, Lei Shi, Wen Xiang Jia, Xiaolin Yin, Jian Yu Su, Yali Kou, Xie Yi, Sumio Shinoda, Shin-ichi Miyoshi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Biofilm formation is an important phenotype associated with chronic Pseudomonas aeruginosa infections. In the present study, a total of 48 P. aeruginosa strains isolated from clinical specimens were examined for their biofilm-forming ability using a microtiter plate method. The different biofilm-forming abilities were demonstrated among the strains; however, most strains formed a larger biofilm than strain PAO1, a reference strain. The genetic typing was also carried out by enterobacterial repetitive intergenic consensus-based polymerase chain reaction. Although they were divided into five groups (A to E), most of the strains showing the higher biofilm-forming ability were found to be in groups D and E, suggesting a significant relationship between the biofilm-forming ability and the genetic group.

Original languageEnglish
Pages (from-to)1057-1061
Number of pages5
JournalMicrobiology and Immunology
Volume49
Issue number12
Publication statusPublished - 2005

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Biofilms
Pseudomonas aeruginosa
Polymerase Chain Reaction
Pseudomonas Infections
Phenotype

Keywords

  • Biofilm
  • Genetic typing
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Evaluation of the biofilm-forming ability and genetic typing for clinical isolates of Pseudomonas aeruginosa by enterobacterial repetitive intergenic consensus-based PCR. / Yang, Weiqing; Shi, Lei; Jia, Wen Xiang; Yin, Xiaolin; Su, Jian Yu; Kou, Yali; Yi, Xie; Shinoda, Sumio; Miyoshi, Shin-ichi.

In: Microbiology and Immunology, Vol. 49, No. 12, 2005, p. 1057-1061.

Research output: Contribution to journalArticle

Yang, Weiqing ; Shi, Lei ; Jia, Wen Xiang ; Yin, Xiaolin ; Su, Jian Yu ; Kou, Yali ; Yi, Xie ; Shinoda, Sumio ; Miyoshi, Shin-ichi. / Evaluation of the biofilm-forming ability and genetic typing for clinical isolates of Pseudomonas aeruginosa by enterobacterial repetitive intergenic consensus-based PCR. In: Microbiology and Immunology. 2005 ; Vol. 49, No. 12. pp. 1057-1061.
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