Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA

Keiki Ogino, Ikuo Murakami, Da Hong Wang, Yorika Tsukiyama, Hidekazu Takahashi, Masayuki Kubo, Noriko Sakano, Heri Setiawan, Masahiko Bando, Yasukazu Ohmoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: We reported previously that serum arginase I increased in asthmatic patients and was associated with oxidative stress in a small healthy population. However, the exact association of arginase I with oxidative stress is not known. The present study aimed to analyze the association of arginase I with oxidative stress in a larger healthy population by a newly established ELISA. Design and methods: The new ELISA for the measurement of human arginase I was established by generating recombinant arginase I protein in human arginase I gene-transfected Escherichia coli via an ARG1 cDNA fragment-inserted vector and -specific antibody in rabbits. Serum arginase I was evaluated in a cross-sectional study on a healthy population (n. = 721) by comparing a commercial ELISA kit with the new ELISA. Results: The mean levels of serum arginase I were 20.3. ±. 0.7. ng/mL and 4.7. ±. 0.2. ng/mL using the commercial ELISA kit and the new ELISA, respectively. Arginase I was correlated with WBC, RBC, hs-CRP, 8-OHdG, HDL-c, ALT, and BMI. Logistic regression analysis showed independent positive associations of arginase I with WBC, RBC, and urinary 8-OHdG and inverse independent associations with serum insulin and age. The association of arginase I with hs-CRP was not independent. Conclusion: The independent associations of arginase I with urinary 8-OHdG and serum insulin may reflect its involvement in oxidative stress and diabetes mellitus.

Original languageEnglish
Pages (from-to)1717-1722
Number of pages6
JournalClinical Biochemistry
Volume46
Issue number16-17
DOIs
Publication statusPublished - Nov 1 2013

    Fingerprint

Keywords

  • Arginase I
  • ELISA
  • Oxidative stress
  • Population study

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Ogino, K., Murakami, I., Wang, D. H., Tsukiyama, Y., Takahashi, H., Kubo, M., Sakano, N., Setiawan, H., Bando, M., & Ohmoto, Y. (2013). Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA. Clinical Biochemistry, 46(16-17), 1717-1722. https://doi.org/10.1016/j.clinbiochem.2013.08.012