Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA

Keiki Ogino, Ikuo Murakami, Da Hong Wang, Yorika Tsukiyama, Hidekazu Takahashi, Masayuki Kubo, Noriko Sakano, Heri Setiawan, Masahiko Bando, Yasukazu Ohmoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: We reported previously that serum arginase I increased in asthmatic patients and was associated with oxidative stress in a small healthy population. However, the exact association of arginase I with oxidative stress is not known. The present study aimed to analyze the association of arginase I with oxidative stress in a larger healthy population by a newly established ELISA. Design and methods: The new ELISA for the measurement of human arginase I was established by generating recombinant arginase I protein in human arginase I gene-transfected Escherichia coli via an ARG1 cDNA fragment-inserted vector and -specific antibody in rabbits. Serum arginase I was evaluated in a cross-sectional study on a healthy population (n. = 721) by comparing a commercial ELISA kit with the new ELISA. Results: The mean levels of serum arginase I were 20.3. ±. 0.7. ng/mL and 4.7. ±. 0.2. ng/mL using the commercial ELISA kit and the new ELISA, respectively. Arginase I was correlated with WBC, RBC, hs-CRP, 8-OHdG, HDL-c, ALT, and BMI. Logistic regression analysis showed independent positive associations of arginase I with WBC, RBC, and urinary 8-OHdG and inverse independent associations with serum insulin and age. The association of arginase I with hs-CRP was not independent. Conclusion: The independent associations of arginase I with urinary 8-OHdG and serum insulin may reflect its involvement in oxidative stress and diabetes mellitus.

Original languageEnglish
Pages (from-to)1717-1722
Number of pages6
JournalClinical Biochemistry
Volume46
Issue number16-17
DOIs
Publication statusPublished - Nov 2013

Fingerprint

Arginase
Oxidative stress
Biomarkers
Oxidative Stress
Enzyme-Linked Immunosorbent Assay
Serum
Population
Association reactions
Insulin
Medical problems
Regression analysis
Escherichia coli
Logistics
Diabetes Mellitus
Complementary DNA
Cross-Sectional Studies
Genes
Logistic Models
Regression Analysis
Rabbits

Keywords

  • Arginase I
  • ELISA
  • Oxidative stress
  • Population study

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA. / Ogino, Keiki; Murakami, Ikuo; Wang, Da Hong; Tsukiyama, Yorika; Takahashi, Hidekazu; Kubo, Masayuki; Sakano, Noriko; Setiawan, Heri; Bando, Masahiko; Ohmoto, Yasukazu.

In: Clinical Biochemistry, Vol. 46, No. 16-17, 11.2013, p. 1717-1722.

Research output: Contribution to journalArticle

Ogino, K, Murakami, I, Wang, DH, Tsukiyama, Y, Takahashi, H, Kubo, M, Sakano, N, Setiawan, H, Bando, M & Ohmoto, Y 2013, 'Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA', Clinical Biochemistry, vol. 46, no. 16-17, pp. 1717-1722. https://doi.org/10.1016/j.clinbiochem.2013.08.012
Ogino, Keiki ; Murakami, Ikuo ; Wang, Da Hong ; Tsukiyama, Yorika ; Takahashi, Hidekazu ; Kubo, Masayuki ; Sakano, Noriko ; Setiawan, Heri ; Bando, Masahiko ; Ohmoto, Yasukazu. / Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA. In: Clinical Biochemistry. 2013 ; Vol. 46, No. 16-17. pp. 1717-1722.
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AU - Takahashi, Hidekazu

AU - Kubo, Masayuki

AU - Sakano, Noriko

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AB - Objective: We reported previously that serum arginase I increased in asthmatic patients and was associated with oxidative stress in a small healthy population. However, the exact association of arginase I with oxidative stress is not known. The present study aimed to analyze the association of arginase I with oxidative stress in a larger healthy population by a newly established ELISA. Design and methods: The new ELISA for the measurement of human arginase I was established by generating recombinant arginase I protein in human arginase I gene-transfected Escherichia coli via an ARG1 cDNA fragment-inserted vector and -specific antibody in rabbits. Serum arginase I was evaluated in a cross-sectional study on a healthy population (n. = 721) by comparing a commercial ELISA kit with the new ELISA. Results: The mean levels of serum arginase I were 20.3. ±. 0.7. ng/mL and 4.7. ±. 0.2. ng/mL using the commercial ELISA kit and the new ELISA, respectively. Arginase I was correlated with WBC, RBC, hs-CRP, 8-OHdG, HDL-c, ALT, and BMI. Logistic regression analysis showed independent positive associations of arginase I with WBC, RBC, and urinary 8-OHdG and inverse independent associations with serum insulin and age. The association of arginase I with hs-CRP was not independent. Conclusion: The independent associations of arginase I with urinary 8-OHdG and serum insulin may reflect its involvement in oxidative stress and diabetes mellitus.

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