Evaluation of Serodiagnosis Detecting IgG Antibodies Against Purified Protein Derivative and Alpha Antigen in Patients with Active Pulmonary Tuberculosis by Enzyme-Linked Immunosorbent Assay

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2 Citations (Scopus)

Abstract

Detection of IgG antibodies against purified protein derivative (PPD) prepared from Mycobacterium tuberculosis strain Aoyama B and alpha antigen, a homogeneous protein prepared from M. tuberculosis strain H37Rv by immunosorbent affinity chromatography, was done by a method of enzyme—linked immunosorbent assay (ELISA) and its diagnostic usefulness was also evaluated in this study. Sera from 80 patients with active pulmonary tuberculosis, 25 patients with inactive tuberculosis, 200 healthy adults, 117 healthy children, 84 patients with diseases other than tuberculosis and seven patients due to atypical mycobacteria were examined. The titer of each test serum was calculated by a computer system using the standard curve obtained by serial dilution of a standard serum. Use of phosphate buffer saline (PBS) with gelatin as blocking buffer in ELISA showed considerably useful results than the use of PBS with bovine serum albumin.

Original languageEnglish
Pages (from-to)211-227
Number of pages17
JournalKekkaku
Volume62
Issue number4
DOIs
Publication statusPublished - Jan 1 1987
Externally publishedYes

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Serologic Tests
Pulmonary Tuberculosis
Immunosorbents
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Antigens
Buffers
Antibodies
Mycobacterium tuberculosis
Tuberculosis
Proteins
Serum
Phosphates
Nontuberculous Mycobacteria
Computer Systems
Gelatin
Bovine Serum Albumin
Affinity Chromatography

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

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abstract = "Detection of IgG antibodies against purified protein derivative (PPD) prepared from Mycobacterium tuberculosis strain Aoyama B and alpha antigen, a homogeneous protein prepared from M. tuberculosis strain H37Rv by immunosorbent affinity chromatography, was done by a method of enzyme—linked immunosorbent assay (ELISA) and its diagnostic usefulness was also evaluated in this study. Sera from 80 patients with active pulmonary tuberculosis, 25 patients with inactive tuberculosis, 200 healthy adults, 117 healthy children, 84 patients with diseases other than tuberculosis and seven patients due to atypical mycobacteria were examined. The titer of each test serum was calculated by a computer system using the standard curve obtained by serial dilution of a standard serum. Use of phosphate buffer saline (PBS) with gelatin as blocking buffer in ELISA showed considerably useful results than the use of PBS with bovine serum albumin.",
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