TY - JOUR
T1 - Evaluation of rapid immunochromatographic tests for norovirus in neonatal and infant faecal specimens
AU - Takahashi, Nobumasa
AU - Nojima, Ikuko
AU - Araki, Tooru
AU - Takasugi, Mizue
AU - Sakane, Tomoko
AU - Kodera, Aya
AU - Ikeda, Masanori
AU - Tsukahara, Hirokazu
N1 - Funding Information:
This study was supported in part by Denka Seiken Co., Ltd.
Publisher Copyright:
© 2015, © The Author(s) 2015.
PY - 2015/10/22
Y1 - 2015/10/22
N2 - Objectives To compare the diagnostic performance of two norovirus rapid immunochromatographic kits (QuickNavi®-Norovirus [QN] and QuickNavi®-Norovirus 2 [QN2]; Denka Seiken, Niigata, Japan) for neonatal and infant faecal specimens. Methods Monthly faecal samples were collected from infants from birth to 12 months of age, and tested for norovirus using QN and QN2. Real-time reverse transcription polymerase chain reaction (RT–PCR) was used as the gold standard for norovirus detection. The diagnostic performance of the kits was calculated. Results A total of 343 specimens from 81 infants were analysed. In all samples, the specificity of QN and QN2 was 80% (275/343) and 99% (339/343), respectively. In infants aged <1 month, the specificity of QN was 33% (23/70), increasing to 93% at 4 months of age. Specificity of QN2 was ≥94% in infants between 0 and 12 months of age. Conclusions QN2 offers improved performance and is more useful than QN for the diagnosis of norovirus infection in the neonatal and infant period.
AB - Objectives To compare the diagnostic performance of two norovirus rapid immunochromatographic kits (QuickNavi®-Norovirus [QN] and QuickNavi®-Norovirus 2 [QN2]; Denka Seiken, Niigata, Japan) for neonatal and infant faecal specimens. Methods Monthly faecal samples were collected from infants from birth to 12 months of age, and tested for norovirus using QN and QN2. Real-time reverse transcription polymerase chain reaction (RT–PCR) was used as the gold standard for norovirus detection. The diagnostic performance of the kits was calculated. Results A total of 343 specimens from 81 infants were analysed. In all samples, the specificity of QN and QN2 was 80% (275/343) and 99% (339/343), respectively. In infants aged <1 month, the specificity of QN was 33% (23/70), increasing to 93% at 4 months of age. Specificity of QN2 was ≥94% in infants between 0 and 12 months of age. Conclusions QN2 offers improved performance and is more useful than QN for the diagnosis of norovirus infection in the neonatal and infant period.
KW - Norovirus
KW - false positive
KW - immunochromatography
KW - neonate
KW - rapid detection test
KW - specificity
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U2 - 10.1177/0300060515592902
DO - 10.1177/0300060515592902
M3 - Article
C2 - 26338763
AN - SCOPUS:84942051697
SN - 0300-0605
VL - 43
SP - 648
EP - 652
JO - Journal of International Medical Research
JF - Journal of International Medical Research
IS - 5
ER -