Evaluation of rapid immunochromatographic tests for norovirus in neonatal and infant faecal specimens

Nobumasa Takahashi, Ikuko Nojima, Tooru Araki, Mizue Takasugi, Tomoko Sakane, Aya Kodera, Masanori Ikeda, Hirokazu Tsukahara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objectives To compare the diagnostic performance of two norovirus rapid immunochromatographic kits (QuickNavi®-Norovirus [QN] and QuickNavi®-Norovirus 2 [QN2]; Denka Seiken, Niigata, Japan) for neonatal and infant faecal specimens. Methods Monthly faecal samples were collected from infants from birth to 12 months of age, and tested for norovirus using QN and QN2. Real-time reverse transcription polymerase chain reaction (RT–PCR) was used as the gold standard for norovirus detection. The diagnostic performance of the kits was calculated. Results A total of 343 specimens from 81 infants were analysed. In all samples, the specificity of QN and QN2 was 80% (275/343) and 99% (339/343), respectively. In infants aged <1 month, the specificity of QN was 33% (23/70), increasing to 93% at 4 months of age. Specificity of QN2 was ≥94% in infants between 0 and 12 months of age. Conclusions QN2 offers improved performance and is more useful than QN for the diagnosis of norovirus infection in the neonatal and infant period.

Original languageEnglish
Pages (from-to)648-652
Number of pages5
JournalJournal of International Medical Research
Volume43
Issue number5
DOIs
Publication statusPublished - Oct 22 2015

Keywords

  • Norovirus
  • false positive
  • immunochromatography
  • neonate
  • rapid detection test
  • specificity

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biochemistry, medical

Fingerprint Dive into the research topics of 'Evaluation of rapid immunochromatographic tests for norovirus in neonatal and infant faecal specimens'. Together they form a unique fingerprint.

  • Cite this